1887

Abstract

is an inhabitant of fructose-rich niches and is a potential member of the fructophilic lactic acid bacteria. In the present study, the phylogenetic and biochemical characteristics of the type strain and eight isolates of , originating from wine, flowers and honey, were studied. The nine isolates, including the type strain, formed a well-defined phylogenetic subcluster based on the analysis of 16S rRNA gene sequences. The subcluster was not closely related to other subclusters in the phylogenetic group. Biochemically, the eight new isolates showed typical fructophilic characteristics. The eight isolates grew poorly on glucose, but grew well on fructose. Good growth on glucose was only recorded in the presence of electron acceptors. The type strain of differed from the other isolates only on the basis of poor growth on fructose. Although they belong to a group of obligately heterofermentative lactic acid bacteria, all nine isolates, including the type strain, produced almost equimolar amounts of lactic acid and acetic acid and very little ethanol from glucose. Eight of the isolates can thus be regarded as typical ‘obligately’ fructophilic lactic acid bacteria. Although the type strain of was phenotypically slightly different from the other isolates, it possessed several important fructophilic characteristics. On the basis of the evidence gathered in this study, the type strain of is recognized as a member of the ‘obligately’ fructophilic lactic acid bacteria.

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2012-03-01
2024-03-28
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References

  1. Akinterinwa O., Khankal R., Cirino P. C. 2008; Metabolic engineering for bioproduction of sugar alcohols. Curr Opin Biotechnol 19:461–467 [View Article][PubMed]
    [Google Scholar]
  2. Bayly J. C. 1999 The isolation, identification and characterization of lactic acid bacteria occurring in South African red wine fermentations. BSc Hons thesis, Stellenbosch University, Stellenbosch.
  3. Edwards C. G., Haag K. M., Collins M. D., Hutson R. A., Huang Y. C. 1998; Lactobacillus kunkeei sp. nov.: a spoilage organism associated with grape juice fermentations. J Appl Microbiol 84:698–702 [View Article][PubMed]
    [Google Scholar]
  4. Endo A., Okada S. 2005; Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55:83–85 [View Article][PubMed]
    [Google Scholar]
  5. Endo A., Okada S. 2006; Oenococcus kitaharae sp. nov., a non-acidophilic and non-malolactic-fermenting oenococcus isolated from a composting distilled shochu residue. Int J Syst Evol Microbiol 56:2345–2348 [View Article][PubMed]
    [Google Scholar]
  6. Endo A., Okada S. 2008; Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov.. Int J Syst Evol Microbiol 58:2195–2205 [View Article][PubMed]
    [Google Scholar]
  7. Endo A., Futagawa-Endo Y., Dicks L. M. T. 2009; Isolation and characterization of fructophilic lactic acid bacteria from fructose-rich niches. Syst Appl Microbiol 32:593–600 [View Article][PubMed]
    [Google Scholar]
  8. Endo A., Futagawa-Endo Y., Sakamoto M., Kitahara M., Dicks L. M. T. 2010; Lactobacillus florum sp. nov., a fructophilic species isolated from flowers. Int J Syst Evol Microbiol 60:2478–2482 [View Article][PubMed]
    [Google Scholar]
  9. Endo A., Irisawa T., Futagawa-Endo Y., Sonomoto K., Itoh K., Takano K., Okada S., Dicks L. M. T. 2011; Fructobacillus tropaeoli sp. nov., a novel fructophilic lactic acid bacterium isolated from a flower. Int J Syst Evol Microbiol 61:898–902 [CrossRef]
    [Google Scholar]
  10. Ezaki T., Yamamoto N., Ninomiya K., Suzuki S., Yabuuchi E. 1983; Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov.. Int J Syst Bacteriol 33:683–698 [View Article]
    [Google Scholar]
  11. Liu S., Saha B., Cotta M. 2005; Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis . Appl Biochem Biotechnol 121:391–401 [View Article][PubMed]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Sasaki Y., Laivenieks M., Zeikus J. G. 2005; Lactobacillus reuteri ATCC 53608 mdh gene cloning and recombinant mannitol dehydrogenase characterization. Appl Microbiol Biotechnol 68:36–41 [View Article][PubMed]
    [Google Scholar]
  14. Taranto M. P., Font de Valdez G., Perez-Martinez G. 1999; Evidence of a glucose proton motive force-dependent permease and a fructose phosphoenolpyruvate:phosphotransferase transport system in Lactobacillus reuteri CRL 1098. FEMS Microbiol Lett 181:109–112 [View Article][PubMed]
    [Google Scholar]
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