@article{mbs:/content/journal/ijsem/10.1099/ijs.0.031419-0, author = "Makhdoumi-Kakhki, A. and Amoozegar, M. A. and Ventosa, A.", title = "Halovenus aranensis gen. nov., sp. nov., an extremely halophilic archaeon from Aran-Bidgol salt lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_6", pages = "1331-1336", doi = "https://doi.org/10.1099/ijs.0.031419-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.031419-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel red-pigmented halophilic archaeon, strain EB27T, was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain EB27T were non-motile and pleomorphic (rods to triangular or disc-shaped). Strain EB27T required at least 2.5 M NaCl and 0.1 M MgCl2 for growth. Optimal growth was achieved at 4 M NaCl and 0.5 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 40 °C; it was able to grow at pH 6.0–8.0 and 25–50 °C. 16S rRNA gene sequence analysis showed that strain EB27T is a member of the family Halobacteriaceae ; however, levels of 16S rRNA gene sequence similarity were as low as 90.0, 89.3 and 89.1 % to the most closely related haloarchaeal taxa, namely Halalkalicoccus tibetensis DS12T, Halosimplex carlsbadense 2-9-1T and Halorhabdus utahensis AX-2T, respectively. The DNA G+C content of strain EB27T was 61 mol%. Strain EB27T contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, together with two minor phospholipids. The only quinone present was MK-8(II-H2). Physiological, biochemical and phylogenetic differences between strain EB27T and recognized genera of extremely halophilic archaea suggest that this strain represents a novel species in a new genus within the family Halobacteriaceae , for which the name Halovenus aranensis gen. nov., sp. nov. is proposed. The type strain of Halovenus aranensis, the type species of the new genus, is strain EB27T ( = IBRC-M 10015T = CGMCC 1.11001T).", }