%0 Journal Article %A Zhai, Lei %A Liao, Tingting %A Xue, Yanfen %A Ma, Yanhe %T Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_4 %P 949-953 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.031575-0 %I Microbiology Society, %X A Gram-positive, alkaliphilic bacterium, designated strain DLS13T, was isolated from Dali Lake in Inner Mongolia Autonomous Region, China. The isolate was able to grow at pH 7.5–11.0 (optimum at pH 9), in 0–8 % (w/v) NaCl (optimum at 2 %, w/v) and at 10–45 °C (optimum at 30 °C). Cells of the isolate were facultatively anaerobic, spore-forming rods with peritrichous flagella. The predominant isoprenoid quinone was MK-7 and its cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0. The genomic DNA G+C content of the isolate was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DLS13T was a member of the genus Bacillus and most closely related to Bacillus saliphilus DSM 15402T (96.9 % similarity). The DNA–DNA relatedness value between strain DLS13T and B. saliphilus DSM 15402T was 38.7±1.9 %. Comparative analysis of genotypic and phenotypic features indicated that strain DLS13T represents a novel species of the genus Bacillus , for which the name Bacillus daliensis sp. nov. is proposed; the type strain is DLS13T ( = CGMCC 1.10369T = JCM 17097T = NBRC 107572T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.031575-0