1887

Abstract

Two novel acetic acid bacteria, strains G5-1 and I5-1, were isolated from traditional kaki vinegar (produced from fruits of kaki, Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains G5-1 and I5-1 formed a distinct subline in the genus and were closely related to DST GL01 (99.3 % 16S rRNA gene sequence similarity). The isolates showed 96–100 % DNA–DNA relatedness with each other, but <53 % DNA–DNA relatedness with closely related members of the genus . The isolates could be distinguished from closely related members of the genus by not producing 2- and 5-ketogluconic acids from glucose, producing cellulose, growing without acetic acid and with 30 % (w/v) -glucose, and producing acid from sugars and alcohols. Furthermore, the genomic DNA G+C contents of strains G5-1 and I5-1 were a little higher than those of their closest phylogenetic neighbours. On the basis of the phenotypic characteristics and phylogenetic position, strains G5-1 and I5-1 are assigned to a novel species, for which the name sp. nov. is proposed; the type strain is G5-1 ( = JCM 25156 = NRIC 0798 = LMG 26206).

Funding
This study was supported by the:
  • Institute for Fermentation, Osaka, Japan (Award 20003-2005)
  • RIKEN, Japan (Award FY2010)
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2012-07-01
2024-04-27
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