1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_7

sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus and , , and Free

Abstract

A Gram-negative, facultatively anaerobic, slightly halophilic bacterium that was motile by gliding, designated KMM 6390, was isolated from the coastal green alga collected from the Sea of Japan. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6390 was a member of the genus , with KMM 6211 as its closest relative (97.8 % 16S rRNA gene sequence similarity). 16S rRNA gene sequence similarities between the isolate and other members of the genus were 95.4–96.7 %. DNA–DNA relatedness between the isolate and KMM 6211 was 27 %. Strain KMM 6390 was able to grow with 0.5–4.0 % NaCl and at 4–37 °C. The isolate decomposed casein, gelatin, starch and DNA and produced acid from galactose, -glucose, lactose and maltose. The major fatty acids were iso-C, iso-C, anteiso-C and C. The polar lipid profile comprised phosphatidylethanolamine and two unknown aminolipids. The DNA G+C content was 34.2 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic investigations, strain KMM 6390 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is KMM 6390 ( = KCTC 23626 = LMG 26444). Emended descriptions of the genus and of , , and are also provided.

  • Published Online:
Funding
This study was supported by the:
  • Russian Foundation for Basic Research (RFBR) (Award grant no. 09-04-00420)
  • Presidium of the Russian Academy of Sciences ‘Molecular and Cell Biology’
  • Government of Russian Federation (Award agreement no. 11.G34.31.0010)
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032219-0
2012-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1450.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032219-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp. 106–111 Edited by Krieg N. J., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;
     [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [View Article][PubMed]
     [Google Scholar]
  4. Collins M. D., Shah H. M. 1984; Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . Arch Microbiol 137:247–249 [CrossRef]
     [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
     [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  7. Felsenstein J. 1993; phylip (phylogeny inference package), version 3.5c. Distributed by the author.. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Ivanova E. P., Christen R., Gorshkova N. M., Zhukova N. V., Kurilenko V. V., Crawford R. J., Mikhailov V. V. 2010; Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella . Int J Syst Evol Microbiol 60:1577–1580 [View Article][PubMed]
     [Google Scholar]
  9. Kim S. B., Nedashkovskaya O. I. 2010; Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 60:1948–1951 [View Article][PubMed]
     [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  11. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
     [Google Scholar]
  12. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  13. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Dobretsov S., Lau K. W. K., Wu M., Wong P.-K., Pawlik J. R., Qian P.-Y. 2005; Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55:1589–1592 [View Article][PubMed]
     [Google Scholar]
  14. Lemos M. L., Toranzo A. E., Barja J. L. 1985; Modified medium for the oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 49:1541–1543[PubMed]
     [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
     [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
     [Google Scholar]
  17. Marshall K., Joint I., Callow M. E., Callow J. A. 2006; Effect of marine bacterial isolates on the growth and morphology of axenic plantlets of the green alga Ulva linza . Microb Ecol 52:302–310 [View Article][PubMed]
     [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Zhukova N. V., Falsen E., Frolova G. M., Mikhailov V. V., Bae K. S. 2003; Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm.. Int J Syst Evol Microbiol 53:1967–1971 [View Article][PubMed]
     [Google Scholar]
  19. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M. S., Lysenko A. M., Falsen E., Frolova G. M., Mikhailov V. V., Bae K. S. 2004; Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 54:119–123 [View Article][PubMed]
     [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Han S. K., Snauwaert C., Vancanneyt M., Swings J., Kim K. O., Lysenko A. M., Rohde M. other authors 2005; Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 55:49–55 [View Article][PubMed]
     [Google Scholar]
  21. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Zhukova N. V. 2009; Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 59:1465–1468 [View Article][PubMed]
     [Google Scholar]
  22. Pinhassi J., Nedashkovskaya O. I., Hagström Å., Vancanneyt M. 2009; Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. Int J Syst Evol Microbiol 59:2180–2184 [View Article][PubMed]
     [Google Scholar]
  23. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2009; Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. Int J Syst Evol Microbiol 59:1443–1446 [View Article][PubMed]
     [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  25. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  26. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
     [Google Scholar]
  27. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  28. Staufenberger T., Thiel V., Wiese J., Imhoff J. F. 2008; Phylogenetic analysis of bacteria associated with Laminaria saccharina . FEMS Microbiol Ecol 64:65–77 [View Article][PubMed]
     [Google Scholar]
  29. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032219-0
Loading
Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia
Int J Syst Evol Microbiol 62, 1450 (2012); https://doi.org/10.1099/ijs.0.032219-0
/content/journal/ijsem/10.1099/ijs.0.032219-0
/content/journal/ijsem/10.1099/ijs.0.032219-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error