1887

Abstract

A re-evaluation of the taxonomic position of five strains, one assigned to (strain 1330, isolated from spiced meat purchased in Slovakia), two previously assigned to genomospecies 1 (strains NCTC 9529 and 731, isolated from water and a leg infection, respectively) and two previously assigned to (strains 96 and 1435, isolated from onion powder and rye flour, respectively) was carried out. The analysis included phenotypic characterization, 16S rRNA gene sequencing and multilocus sequence analysis (MLSA) of seven housekeeping genes (, , , , , , ; 3036 bp). 16S rRNA gene sequence analysis and MLSA showed that strain 1330 formed an independent phylogenetic lineage in the MLSA, with LMG 23823 as the closest neighbour. DNA–DNA reassociation and phenotypic analysis revealed that strain 1330 represented a novel species, for which the name sp. nov. is proposed (type strain 1330 = CECT 7863 = LMG 26250). Strains NCTC 9529, 731, 96 and 1435 clustered together within an independent phylogenetic lineage, with LMG 23827 as the closest neighbour in the MLSA. DNA–DNA reassociation and phenotypic analysis confirmed that these strains represent a novel species, for which the name sp. nov. is proposed (type strain NCTC 9529 = CECT 7864 = LMG 26249).

Funding
This study was supported by the:
  • Nottingham Trent University
  • Wellcome Trust
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2012-06-01
2024-03-28
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References

  1. Baldwin A., Loughlin M., Caubilla-Barron J., Kucerova E., Manning G., Dowson C., Forsythe S. 2009; Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes. BMC Microbiol 9:223 [View Article][PubMed]
    [Google Scholar]
  2. British Society for Antimicrobial Chemotherapy 2010; BSAC methods for antimicrobial susceptibility testing, version 9.. http://www.bsac.org.uk/Resources/BSAC/Version_9.1_March_2010_final.pdf
  3. Caubilla-Barron J., Hurrell E., Townsend S., Cheetham P., Loc-Carrillo C., Fayet O., Prère M.-F., Forsythe S. J. 2007; Genotypic and phenotypic analysis of Enterobacter sakazakii strains from an outbreak resulting in fatalities in a neonatal intensive care unit in France. J Clin Microbiol 45:3979–3985 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. . 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Duaga C., Breuwer P. 2008; Taxonomy and physiology of Enterobacter sakazakii . In Enterobacter sakazakii pp. 1–26 Edited by Farber J. M., Forsythe S. J. Washington, DC: American Society for Microbiology; [CrossRef]
    [Google Scholar]
  6. Farmer J. J. III, Asbury M. A., Hickman F. W., Brenner D. J. Enterobacteriaceae study group 1980; Enterobacter sakazakii: a new species of ‘Enterobacteriaceae’ isolated from clinical specimens. Int J Syst Bacteriol 30:569–584 [View Article]
    [Google Scholar]
  7. Forsythe S. J. 2005; Enterobacter sakazakii and other bacteria in powdered infant milk formula. Matern Child Nutr 1:44–50 [View Article][PubMed]
    [Google Scholar]
  8. Harayama S., Kasai H. 2006; Bacterial phylogeny reconstruction from molecular sequences. In Molecular Identification, Systematics, and Population Structure of Prokaryotes pp. 105–140 Edited by Stackebrandt E. Berlin, Heidelberg: Springer-Verlag.; [CrossRef]
    [Google Scholar]
  9. Ibarz Pavón A. B., Maiden M. C. 2009; Multilocus sequence typing. Methods Mol Biol 551:129–140 [View Article][PubMed]
    [Google Scholar]
  10. Iversen C., Forsythe S. J. 2004; Isolation of Enterobacter sakazakii and other Enterobacteriaceae from powdered infant formula milk and related products. Food Microbiol 21:771–777 [View Article]
    [Google Scholar]
  11. Iversen C., Waddington M., On S. L. W., Forsythe S. 2004; Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J Clin Microbiol 42:5368–5370 [View Article][PubMed]
    [Google Scholar]
  12. Iversen C., Lancashire L., Waddington M., Forsythe S., Ball G. 2006a; Identification of Enterobacter sakazakii from closely related species: the use of artificial neural networks in the analysis of biochemical and 16S rDNA data. BMC Microbiol 6:28 [View Article][PubMed]
    [Google Scholar]
  13. Iversen C., Waddington M., Farmer J. J. III, Forsythe S. J. 2006b; The biochemical differentiation of Enterobacter sakazakii genotypes. BMC Microbiol 6:94 [View Article][PubMed]
    [Google Scholar]
  14. Iversen C., Lehner A., Mullane N., Bidlas E., Cleenwerck I., Marugg J., Fanning S., Stephan R., Joosten H. 2007; The taxonomy of Enterobacter sakazakii: proposal of a new genus Cronobacter gen. nov. and descriptions of Cronobacter sakazakii comb. nov. Cronobacter sakazakii subsp. sakazakii, comb. nov., Cronobacter sakazakii subsp. malonaticus subsp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov. and Cronobacter genomospecies 1. BMC Evol Biol 7:64 [View Article][PubMed]
    [Google Scholar]
  15. Iversen C., Mullane N., McCardell B., Tall B. D., Lehner A., Fanning S., Stephan R., Joosten H. 2008; Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.. Int J Syst Evol Microbiol 58:1442–1447 [View Article][PubMed]
    [Google Scholar]
  16. Jolley K. A., Chan M.-S., Maiden M. C. J. 2004; mlstdbNet – distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5:86 [View Article][PubMed]
    [Google Scholar]
  17. Joseph S., Forsythe S. J. 2011; Predominance of Cronobacter sakazakii sequence type 4 in neonatal infections. Emerg Infect Dis 17:1713–1715 [View Article][PubMed]
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  19. Kucerova E., Clifton S. W., Xia X.-Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K. other authors 2010; Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species. PLoS One 5:e9556 [View Article][PubMed]
    [Google Scholar]
  20. Kucerova E., Joseph S., Forsythe S. 2011; The Cronobacter genus: ubiquity and diversity. Quality Assurance and Safety of Crops and Foods 3:104–122 [CrossRef]
    [Google Scholar]
  21. Lacher D. W., Steinsland H., Blank T. E., Donnenberg M. S., Whittam T. S. 2007; Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. J Bacteriol 189:342–350 [View Article][PubMed]
    [Google Scholar]
  22. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. other authors 2007; clustal w and clustal x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  24. Osaili T., Forsythe S. 2009; Desiccation resistance and persistence of Cronobacter species in infant formula. Int J Food Microbiol 136:214–220 [View Article][PubMed]
    [Google Scholar]
  25. Rosselló-Mora R. 2006; DNA–DNA reassociation methods applied to microbial taxonomy and their critical evaluation. In Molecular Identification, Systematics, and Population Structure of Prokaryotes pp. 23–50 Edited by Stackebrandt E. Berlin: Springer; [View Article]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  29. Townsend S. M., Hurrell E., Caubilla-Barron J., Loc-Carrillo C., Forsythe S. J. 2008; Characterization of an extended spectrum beta-lactamase Enterobacter hormaechei nosocomial outbreak, and other Enterobacter hormaechei misidentified as Cronobacter (Enterobacter)sakazakii . Microbiology 154:3659–3667 [View Article][PubMed]
    [Google Scholar]
  30. Turcovský I., Kuniková K., Drahovská H., Kaclíková E. 2011; Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods. Antonie van Leeuwenhoek 99:257–269 [View Article][PubMed]
    [Google Scholar]
  31. Urdiain M., López-López A., Gonzalo C., Busse H. J., Langer S., Kämpfer P., Rosselló-Móra R. 2008; Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . Syst Appl Microbiol 31:339–351 [View Article][PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
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