1887

Abstract

A novel, facultatively anaerobic bacterium (strain JAM-BA0501) was isolated from a deep subseafloor sediment sample at a depth of 247 m below seafloor off the Shimokita Peninsula of Japan in the north-western Pacific Ocean (Site C9001, 1180 m water depth). Cells of strain JAM-BA0501 were Gram-negative, filamentous, non-spore-forming and motile on solid medium by gliding. Phylogenetic analysis based on the 16S rRNA gene sequence of strain JAM-BA0501 indicated a distant relationship to strains representing genera within the order , such as Z-7010 (91.1 % similarity), ATCC 19041 (86.2 %) and Fru22 (89.3 %). The new isolate produced isoprenoid quinones with menaquinone MK-7 as the major component, and the predominant fatty acids were iso-C and anteiso-C. The DNA G+C content of the isolate was 42.9 mol%. Based on its taxonomic distinctiveness, strain JAM-BA0501 is considered to represent a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is JAM-BA0501 ( = JCM 15548  = NCIMB 14482).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032326-0
2012-05-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/5/1075.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032326-0&mimeType=html&fmt=ahah

References

  1. Bachmann B. J. 1955; Studies on Cytophaga fermentans, n.sp., a facultatively anaerobic lower myxobacterium. J Gen Microbiol 13:541–551[PubMed] [CrossRef]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  3. Denger K., Warthmann R., Ludwig W., Schink B. 2002; Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium. Int J Syst Evol Microbiol 52:173–178[PubMed]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  5. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. (editors) 1994 Bergey’s Manual of Determinative Bacteriology, 9th edn. Baltimore: Williams and Wilkins;
    [Google Scholar]
  6. Kobayashi T., Koide O., Mori K., Shimamura S., Matsuura T., Miura T., Takaki Y., Morono Y., Nunoura T. other authors 2008; Phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off Shimokita Peninsula. Extremophiles 12:519–527 [View Article][PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  8. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  9. Ludwig W., Euzeby J., Whitman W. B. 2008 Draft Taxonomic Outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. Published online: http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
  10. MIDI 1999 Sherlock, Microbial Identification System, Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  11. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for extracting bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  12. Miyazaki M., Nogi Y., Fujiwara Y., Kawato M., Kubokawa K., Horikoshi K. 2008; Neptunomonas japonica sp. nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan. Int J Syst Evol Microbiol 58:866–871 [View Article][PubMed]
    [Google Scholar]
  13. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y. 2010; Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. Int J Syst Evol Microbiol 60:2298–2302 [View Article][PubMed]
    [Google Scholar]
  14. Na H., Kim S., Moon E. Y., Chun J. 2009; Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59:2241–2246 [View Article][PubMed]
    [Google Scholar]
  15. Nakagawa Y., Yamasato K. 1996; Emendation of the genus Cytophaga and transfer of Cytophaga agarovorans and Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium–Cytophaga complex. Int J Syst Bacteriol 46:599–603 [View Article]
    [Google Scholar]
  16. Nogi Y., Kato C. 1999; Taxonomic studies of extremely barophilic bacteria isolated from the Mariana Trench and description of Moritella yayanosii sp. nov., a new barophilic bacterial isolate. Extremophiles 3:71–77 [View Article][PubMed]
    [Google Scholar]
  17. Nogi Y., Kato C., Horikoshi K. 1998; Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov.. Arch Microbiol 170:331–338 [View Article][PubMed]
    [Google Scholar]
  18. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 1999; Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. Int J Syst Bacteriol 49:1551–1557 [View Article][PubMed]
    [Google Scholar]
  21. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates;
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  23. Veldkamp H. 1961; A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J Gen Microbiol 26:331–342[PubMed] [CrossRef]
    [Google Scholar]
  24. Zhilina T. N., Appel R., Probian C., Brossa E. L., Harder J., Widdel F., Zavarzin G. A. 2004; Alkaliflexus imshenetskii gen. nov. sp. nov., a new alkaliphilic gliding carbohydrate-fermenting bacterium with propionate formation from a soda lake. Arch Microbiol 182:244–253 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032326-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032326-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error