Massilia oculi sp. nov., isolated from a human clinical specimen
Kämpfer, Peter and Lodders, Nicole and Martin, Karin and Falsen, Enevold,, 62, 364-369 (2012),
doi = https://doi.org/10.1099/ijs.0.032441-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-negative, rod-shaped, non-spore-forming bacterium (strain CCUG 43427AT) was isolated from a patient suffering from endophthalmitis and its taxonomic position was studied. 16S rRNA gene sequence analysis indicated that this strain was a member of the genus Massilia. Strain CCUG 43427AT was most closely related to the type strains of Massilia timonae (97.4 % 16S rRNA gene sequence similarity) and Massilia aurea (97.2 %); levels of similarity to the type strains of all other recognized Massilia species were below 97.0 %. Chemotaxonomic data [Q-8 as major ubiquinone; phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as major polar lipids; and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1ω7c, C12 : 0 and C10 : 0 3-OH as major fatty acids] supported the affiliation of the isolate to the genus Massilia. Levels of DNA–DNA relatedness of strain CCUG 43427AT with M. timonae CCUG 45783T and M. aurea AP13T were 60.6 % (reciprocal, 55.8 %) and 58.1 % (reciprocal, 34.0 %), respectively. Strain CCUG 43427AT could be differentiated from its closest phylogenetic neighbours based on a range of phenotypic characteristics. Strain CCUG 43427AT is therefore considered to represent a novel species of the genus Massilia, for which the name Massilia oculi sp. nov. is proposed. The type strain is CCUG 43427AT ( = CCM 7900T).,
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