RT Journal Article SR Electronic(1) A1 Jabari, Linda A1 Gannoun, Hana A1 Cayol, Jean-Luc A1 Hedi, Abdeljabbar A1 Sakamoto, Mitsuo A1 Falsen, Enevold A1 Ohkuma, Moriya A1 Hamdi, Moktar A1 Fauque, Guy A1 Ollivier, Bernard A1 Fardeau, Marie-LaureYR 2012 T1 Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_10 SP 2522 OP 2527 DO https://doi.org/10.1099/ijs.0.032508-0 PB Microbiology Society, SN 1466-5034, AB A novel obligately anaerobic, non-spore-forming, rod-shaped mesophilic bacterium, which stained Gram-positive but showed the typical cell wall structure of Gram-negative bacteria, was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. The strain, designated LIND7HT, grew at 20–45 °C (optimum 35–40 °C) and at pH 5.0–8.5 (optimum pH 6.5–7.5). It did not require NaCl for growth, but was able to grow in the presence of up to 2 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain LIND7HT used cellobiose, glucose, lactose, mannose, maltose, peptone, rhamnose, raffinose, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were lactate, acetate, butyrate and isobutyrate. The predominant cellular fatty acids were anteiso-C15 : 0, C15 : 0, C17 : 0 2-OH and a summed feature consisting of C18 : 2ω6,9c and/or anteiso-C18 : 0, and the major menaquinones were MK-9, MK-9(H2) and MK-10. The G+C content of the genomic DNA was 41.4 mol%. Although the closest phylogenetic relatives of strain LIND7HT were Parabacteroides merdae , Parabacteroides goldsteinii and Parabacteroides gordonii , analysis of the hsp60 gene sequence showed that strain LIND7HT was not a member of the genus Parabacteroides . On the basis of phylogenetic inference and phenotypic properties, strain LIND7HT ( = CCUG 60892T = DSM 23697T = JCM 16313T) is proposed as the type strain of a novel species in a new genus within the family Porphyromonadaceae , Macellibacteroides fermentans gen. nov., sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.032508-0