1887

Abstract

An orange, rod-shaped, gliding bacterium, designated strain PSC33, was isolated from the gut microflora of a mussel collected from Gwangyang Bay, South Sea (Republic of Korea). Cells were Gram-reaction-negative, strictly aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C, iso-C 3-OH, iso-C G, C 3-OH and iso-Cω9. The only isoprenoid quinone was menaquinone-6 (MK-6). The DNA G+C content of strain PSC33 was 37.9 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain PSC33 formed an evolutionary lineage within the radiation encompassing members of the genus with JAMB N27 as its nearest neighbour (96.3 % sequence similarity). A number of phenotypic characteristics distinguished strain PSC33 from recognized members of the genus . On the basis of the data presented in this study, strain PSC33 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PSC33 ( = KCTC 23302 = JCM 17454). An emended description of is also provided.

Funding
This study was supported by the:
  • Ministry of Education, Science & Technology
  • Ministry of Environment, Republic of Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032904-0
2012-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1974.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032904-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. CLSI 2009 Performance standards for antimicrobial susceptibility testing; 19th Informational Supplement, CLSI document M100-S19 (ISBN 1-56238-690-5). Wayne, PA: Clinical and Laboratory Standards Institute.
  6. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp. 265–309 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  10. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [View Article][PubMed]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  13. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  14. Lewin R. A. 1969; A classification of flexibacteria. J Gen Microbiol 58:189–206[PubMed] [CrossRef]
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  16. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:1055–1063 [View Article][PubMed]
    [Google Scholar]
  17. MIDI 1999 Sherlock Microbial Identification System Operating Manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  19. Miyazaki M., Nagano Y., Fujiwara Y., Hatada Y., Nogi Y. 2010; Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses. Int J Syst Evol Microbiol 60:2298–2302 [View Article][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005; Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.. Int J Syst Evol Microbiol 55:225–229 [View Article][PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Vancanneyt M., Christiaens L., Kalinovskaya N. I., Mikhailov V. V., Swings J. 2006; Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina . Int J Syst Evol Microbiol 56:2037–2041 [View Article][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; General characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates;
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  26. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Yang S.-J., Cho J.-C. 2008; Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 58:315–318 [View Article][PubMed]
    [Google Scholar]
  29. Yang S.-H., Kwon K. K., Lee H.-S., Kim S.-J. 2006; Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56:2879–2882 [View Article][PubMed]
    [Google Scholar]
  30. Yi H., Chun J. 2011; Aquimarina addita sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:2445–2449 [View Article][PubMed]
    [Google Scholar]
  31. Yoon J. H., Kang S. J., Jung S. Y., Oh H. W., Oh T. K. 2006; Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 56:115–119 [View Article][PubMed]
    [Google Scholar]
  32. Yoon B. J., You H. S., Lee D. H., Oh D. C. 2011; Aquimarina spongiae sp. nov., isolated from marine sponge Halichondria oshoro . Int J Syst Evol Microbiol 61:417–421 [View Article][PubMed]
    [Google Scholar]
  33. Yumoto I., Hirota K., Yamaga S., Nodasaka Y., Kawasaki T., Matsuyama H., Nakajima K. 2004; Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54:1997–2001 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032904-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032904-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error