@article{mbs:/content/journal/ijsem/10.1099/ijs.0.032912-0, author = "Ribeiro, Renan Augusto and Rogel, Marco A. and López-López, Aline and Ormeño-Orrillo, Ernesto and Barcellos, Fernando Gomes and Martínez, Julio and Thompson, Fabiano Lopes and Martínez-Romero, Esperanza and Hungria, Mariangela", title = "Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_5", pages = "1179-1184", doi = "https://doi.org/10.1099/ijs.0.032912-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.032912-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = " Rhizobium tropici is a well-studied legume symbiont characterized by high genetic stability of the symbiotic plasmid and tolerance to tropical environmental stresses such as high temperature and low soil pH. However, high phenetic and genetic variabilities among R. tropici strains have been largely reported, with two subgroups, designated type A and B, already defined within the species. A polyphasic study comprising multilocus sequence analysis, phenotypic and genotypic characterizations, including DNA–DNA hybridization, strongly supported the reclassification of R. tropici type A strains as a novel species. Type A strains formed a well-differentiated clade that grouped with R. tropici , Rhizobium multihospitium , Rhizobium miluonense , Rhizobium lusitanum and Rhizobium rhizogenes in the phylogenies of the 16S rRNA, recA, gltA, rpoA, glnII and rpoB genes. Several phenotypic traits differentiated type A strains from all related taxa. The novel species, for which the name Rhizobium leucaenae sp. nov. is proposed, is a broad host range rhizobium being able to establish effective root-nodule symbioses with Leucaena leucocephala, Leucaena esculenta, common beans (Phaseolus vulgaris) and Gliricidia sepium. Strain CFN 299T ( = USDA 9039T = LMG 9517T = CECT 4844T = JCM 21088T = IAM 14230T = SEMIA 4083T = CENA 183T = UMR1026T = CNPSo 141T) is designated the type strain of Rhizobium leucaenae sp. nov.", }