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Abstract

A bacterial strain (B2-7) capable of degrading a wide range of polycyclic aromatic hydrocarbon compounds (2–4 rings) was isolated from a water sample taken from Botan Oil Port in Xiamen, China. The isolate was Gram-negative, short-rod-shaped, aerobic, non-motile and formed yellow-pigmented colonies on LB medium. Cells of strain B2-7 were catalase-positive and oxidase-negative. Optimal growth of strain B2-7 was observed at pH 7.0, at 26 °C and in 0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain B2-7 grouped with members of the genus and it showed 16S rRNA gene sequence similarity of 95.40 % to YIM 003. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. Q-10 and -homospermidine were the predominant ubiquinone and polyamine components, respectively. The major fatty acids were Cω7 (67.2 %), C 2-OH (10.0 %) and C (9.6 %). The G+C content of the genomic DNA was 61.8 mol%. Based on phenotypic properties, and phylogenetic and genomic data, strain B2-7 represents a novel species of the genus within the class , for which the name sp. nov. is proposed. The type strain is B2-7 ( = KCCM 42951 = JCM 16711).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30710103908, 40930847 and 40976069)
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2012-06-01
2024-03-19
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References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  2. Busse J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  3. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Evol Microbiol 47:698–708
    [Google Scholar]
  4. Busse H. J., Kämpfer P., Denner E. B. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  7. Collins M. D., Goodfellow M., Minnikin D. E. 1980; Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118:29–37[PubMed]
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Geueke B., Busse H.-J., Fleischmann T., Kämpfer P., Kohler H.-P. E. 2007; Description of Sphingosinicella xenopeptidilytica sp. nov., a beta-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans . Int J Syst Evol Microbiol 57:107–113 [View Article][PubMed]
    [Google Scholar]
  10. Huang X., Tian Y., Luo Y. R., Liu H. J., Zheng W., Zheng T. L. 2008; Modified sublimation to isolate phenanthrene-degrading bacteria of the genera Sphingomonas and Burkholderia from Xiamen oil port. Mar Pollut Bull 57:538–543 [View Article][PubMed]
    [Google Scholar]
  11. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic-acid by high-performance liquid-chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  13. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [View Article]
    [Google Scholar]
  14. Pinyakong O., Habe H., Omori T. 2003; The unique aromatic catabolic genes in sphingomonads degrading polycyclic aromatic hydrocarbons (PAHs). J Gen Appl Microbiol 49:1–19 [View Article][PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  16. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Stolz A. 2009; Molecular characteristics of xenobiotic-degrading sphingomonads. Appl Microbiol Biotechnol 81:793–811 [View Article][PubMed]
    [Google Scholar]
  18. Stolz A., Busse H. J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
    [Google Scholar]
  19. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417[PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  21. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119[PubMed] [CrossRef]
    [Google Scholar]
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