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Volume 62, Issue Pt_8

sp. nov., a nematicidal endophytic bacterium isolated from tobacco Free

Abstract

A novel Gram-negative strain, designated M-SX103, was isolated from a fresh leaf of Linn. collected at Yuxi City, Yunnan province, China, and subjected to a polyphasic taxonomic study. Growth occurred at 10–40 °C, at pH 6.0–9.0 and with 0–3 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids of strain M-SX103 were iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6). The G+C content of the genomic DNA was 40.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus , sharing 95.4–89.7 % sequence similarity with type strains of members of the genus ; it was most closely related to T5-12. On the basis of the taxonomic evidence, a novel species, sp. nov., is proposed. The type strain is M-SX103 ( = JCM 17339 = CCTCC AB 2010390).

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Funding
This study was supported by the:
  • National Key Sciences and Technology Program for Water Solutions (Award 2012ZX07102-003)
  • NSFC (Award 30970100, 31160376)
  • ZhengZhou Tobacco Research Institute (Award 122009CZ0420)
  • Yunnan Corporation (Award 2011YN03)
  • CNTC, Yunnan University (Award 2009C16Q)
  • Special Fund of Green Agricultural Biotechnology Products of National Development and Reform Commission
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2012-08-01
2024-04-23
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  2. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [View Article]
     [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
     [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  5. Duan S., Liu Z., Feng X., Zheng K., Cheng L. 2009; Sphingobacterium bambusae sp. nov., isolated from soil of bamboo plantation. J Microbiol 47:693–698 [View Article][PubMed]
     [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  7. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  9. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
     [Google Scholar]
  10. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  11. He X., Xiao T., Kuang H., Lan X., Tudahong M., Osman G., Fang C., Rahman E. 2010; Sphingobacterium shayense sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 60:2377–2381 [View Article][PubMed]
     [Google Scholar]
  12. Kim K.-H., Ten L. N., Liu Q.-M., Im W.-T., Lee S.-T. 2006; Sphingobacterium daejeonense sp. nov., isolated from a compost sample. Int J Syst Evol Microbiol 56:2031–2036 [View Article][PubMed]
     [Google Scholar]
  13. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
     [Google Scholar]
  14. Liu R., Liu H., Zhang C.-X., Yang S.-Y., Liu X.-H., Zhang K.-Y., Lai R. 2008; Sphingobacterium siyangense sp. nov., isolated from farm soil. Int J Syst Evol Microbiol 58:1458–1462 [View Article][PubMed]
     [Google Scholar]
  15. Matsuyama H., Katoh H., Ohkushi T., Satoh A., Kawahara K., Yumoto I. 2008; Sphingobacterium kitahiroshimense sp. nov., isolated from soil. Int J Syst Evol Microbiol 58:1576–1579 [View Article][PubMed]
     [Google Scholar]
  16. Mehnaz S., Weselowski B., Lazarovits G. 2007; Sphingobacterium canadense sp. nov., an isolate from corn roots. Syst Appl Microbiol 30:519–524 [View Article][PubMed]
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  20. Shivaji S., Ray M. K., Shyamala Rao N., Saisree L., Jagannadham M. V., Seshu Kumar G., Reddy G. S. N., Bhargava P. M. 1992; Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–106 [View Article]
     [Google Scholar]
  21. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov.. Int J Syst Bacteriol 48:165–177 [View Article][PubMed]
     [Google Scholar]
  22. Takeuchi M., Yokota A. 1992; Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium, and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum . J Gen Appl Microbiol 38:465–482 [View Article]
     [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  24. Ten L. N., Liu Q.-M., Im W.-T., Aslam Z., Lee S.-T. 2006; Sphingobacterium composti sp. nov., a novel DNase-producing bacterium isolated from compost. J Microbiol Biotechnol 16:1728–1733
     [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  26. Wei W., Zhou Y., Wang X., Huang X., Lai R. 2008; Sphingobacterium anhuiense sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 58:2098–2101 [View Article][PubMed]
     [Google Scholar]
  27. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. 1983; Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov. glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33:580–598 [View Article]
     [Google Scholar]
  28. Zhang J., Zheng J.-W., Cho B. C., Hwang C. Y., Fang C., He J., Li S.-P. 2012; Sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge. Int J Syst Evol Microbiol 62:683–687 [View Article][PubMed]
     [Google Scholar]
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Sphingobacterium nematocida sp. nov., a nematicidal endophytic bacterium isolated from tobacco
Int J Syst Evol Microbiol 62, 1809 (2012); https://doi.org/10.1099/ijs.0.033670-0
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