RT Journal Article SR Electronic(1) A1 Ben Hania, Wajdi A1 Godbane, Ramzi A1 Postec, Anne A1 Hamdi, Moktar A1 Ollivier, Bernard A1 Fardeau, Marie-LaureYR 2012 T1 Defluviitoga tunisiensis gen. nov., sp. nov., a thermophilic bacterium isolated from a mesothermic and anaerobic whey digester JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_6 SP 1377 OP 1382 DO https://doi.org/10.1099/ijs.0.033720-0 PB Microbiology Society, SN 1466-5034, AB Strain SulfLac1T, a thermophilic, anaerobic and slightly halophilic, rod-shaped bacterium with a sheath-like outer structure (toga), was isolated from a whey digester in Tunisia. The strain’s non-motile cells measured 3–30×1 µm and appeared singly, in pairs or as long chains. The novel strain reduced thiosulfate and elemental sulfur, but not sulfate or sulfite, into sulfide. It grew at 37–65 °C (optimum 55 °C), at pH 6.5–7.9 (optimum pH 6.9) and with 0.2–3 % (w/v) NaCl (optimum 0.5 %). The G+C content of the strain’s genomic DNA was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SulfLac1T was most closely related to Petrotoga mobilis (91.4 % sequence similarity). Based on phenotypic, phylogenetic and chemotaxonomic evidence, strain SulfLac1T represents a novel species of a new genus within the order Thermotogales , for which the name Defluviitoga tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is SulfLac1T ( = DSM 23805T  = JCM 17210T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.033720-0