1887

Abstract

A Gram-staining-positive, moderately halophilic bacterium, designated strain BH097, was isolated from solar saltern soil of Bigeum Island in south-west Korea. Cells were motile rods, producing spherical endospores at a terminal position in swollen sporangia. Strain BH097 was strictly aerobic, grew at pH 5.5–9.5 (optimum, pH 8.0), at 10–52 °C (optimum, 37 °C) and at salinities of 1–22 % (w/v) NaCl (optimum, 7 % NaCl). On the basis of 16S rRNA gene sequence analysis, strain BH097 was shown to belong to the genus within the phylum , and showed closest sequence similarity to DSM 19802 (95.8 %), TP2-8 (95.6 %), DSM 17256 (95.5 %), ‘’ DSM 17858 (95.4 %) and DSM 17856 (95.2 %). The DNA G+C content of this novel isolate was 37.9 mol%. The major cellular fatty acids of strain BH097 were anteiso-C, iso-C and C, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol two unknown phospholipids and a glycolipid. The isoprenoid quinone was MK-7, and the peptidoglycan type was A1γ, with -diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, strain BH097 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is BH097 ( = KCTC 13130 = DSM 19028).

Funding
This study was supported by the:
  • Korean Ministry of Education, Science & Technology (MEST)
  • KRIBB Research Initiative Program, Korea
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.034264-0
2012-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1857.html?itemId=/content/journal/ijsem/10.1099/ijs.0.034264-0&mimeType=html&fmt=ahah

References

  1. Ahmed I., Yokota A., Fujiwara T. 2007; Gracilibacillus boraciitolerans sp. nov., a highly boron-tolerant and moderately halotolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57:796–802 [View Article][PubMed]
    [Google Scholar]
  2. Arahal D. R., Ventosa A. 2002; Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives pp. 83–99 Edited by Berkeley R., Heyndrickx M., Logan N., De Vos P. Oxford: Blackwell; [View Article]
    [Google Scholar]
  3. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  4. Carrasco I. J., Márquez M. C., Yanfen X., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. 2006; Gracilibacillus orientalis sp. nov., a novel moderately halophilic bacterium isolated from a salt lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56:599–604 [View Article][PubMed]
    [Google Scholar]
  5. Chamroensaksri N., Tanasupawat S., Akaracharanya A., Visessanguan W., Kudo T., Itoh T. 2010; Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra). Int J Syst Evol Microbiol 60:944–948 [View Article][PubMed]
    [Google Scholar]
  6. Chen Y.-G., Cui X.-L., Zhang Y.-Q., Li W.-J., Wang Y.-X., Xu L.-H., Peng Q., Wen M.-L., Jiang C.-L. 2008a; Gracilibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from saline soil. Int J Syst Evol Microbiol 58:2403–2408 [View Article][PubMed]
    [Google Scholar]
  7. Chen Y.-G., Cui X.-L., Zhang Y.-Q., Li W.-J., Wang Y.-X., Xu L.-H., Peng Q., Wen M.-L., Jiang C.-L. 2008b; Gracilibacillus quinghaiensis sp. nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China. Syst Appl Microbiol 31:183–189 [View Article][PubMed]
    [Google Scholar]
  8. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  10. DeLong E. F. 1992; Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89:5685–5689 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  13. Gomori G. 1955; Preparation of buffers for use in enzyme studies. In Methods in Enzymology vol. 1 pp. 138–146 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press; [View Article]
    [Google Scholar]
  14. Huo Y. Y., Xu X. W., Cui H. L., Wu M. 2010; Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. Int J Syst Evol Microbiol 60:1383–1386 [View Article][PubMed]
    [Google Scholar]
  15. Jeon C. O., Lim J.-M., Jang H. H., Park D.-J., Xu L.-H., Jiang C.-L., Kim C.-J. 2008; Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China. Int J Syst Evol Microbiol 58:2282–2286 [View Article][PubMed]
    [Google Scholar]
  16. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  17. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
    [Google Scholar]
  18. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  19. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  20. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. other authors 2007; clustal w and clustal_x version 2.0. Bioinformatics 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  21. Lawson P. A., Deutch C. E., Collins M. D. 1996; Phylogenetic characterization of a novel salt-tolerant Bacillus species: description of Bacillus dipsosauri sp. nov.. J Appl Bacteriol 81:109–112 [View Article][PubMed]
    [Google Scholar]
  22. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  23. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  24. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  27. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  28. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  29. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  30. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  31. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A., Kämpfer P., Maiden M. C., Nesme X., Rosselló-Mora R., Swings J. other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [View Article][PubMed]
    [Google Scholar]
  32. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  33. Tang S.-K., Wang Y., Lou K., Mao P.-H., Jin X., Jiang C.-L., Xu L.-H., Li W.-J. 2009; Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 59:1620–1624 [View Article][PubMed]
    [Google Scholar]
  34. Ventosa A. 2006; Unusual micro-organisms from unusual habitats: hypersaline environments. In Prokaryotic Diversity – Mechanisms and Significance, (Society for General Microbiology Symposium no. 66) pp. 223–254 Edited by Logan N. A., Lappin-Scott H. M., Oyston P. C. F. Cambridge: Cambridge University Press;
    [Google Scholar]
  35. Wainø M., Tindall B. J., Schumann P., Ingvorsen K. 1999; Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:821–831 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.034264-0
Loading
/content/journal/ijsem/10.1099/ijs.0.034264-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error