1887

Abstract

A symbiotic bacterium, strain IMI 397775, was isolated from the insect-pathogenic nematode . On the basis of 16S rRNA gene sequence similarity, this bacterial isolate was shown to belong to the genus , in agreement with the genus of its nematode host. The accurate phylogenetic position of this new isolate was defined using a multigene approach and showed that isolate IMI 397775 shares a common ancestor with FRM16 and PR06-A, the symbiotic bacteria associated with and , respectively. The nucleotide identity (less than 97 %) between isolate IMI 397775, FRM16 and PR06-A calculated for the concatenated sequences of five gene fragments encompassing 4275 nt, several phenotypic traits and the difference between the upper temperatures that limit growth of these three bacteria allowed genetic and phenotypic differentiation of isolate IMI 397775 from the two closely related species. Strain IMI 397775 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain IMI 397775 ( = DSM 24915).

Funding
This study was supported by the:
  • Darwin Initiative (Award 15/004)
  • CABI
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2012-08-01
2024-03-29
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References

  1. Akhurst R. J. 1980; Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis . J Gen Microbiol 121:303–309
    [Google Scholar]
  2. Akhurst R. J., Boemare N. E. 1988; A numerical taxonomic study of the genus Xenorhabdus (Enterobacteriaceae) and proposed elevation of the subspecies of X. nematophilus to species. J Gen Microbiol 134:1835–1845[PubMed]
    [Google Scholar]
  3. Akhurst R. J., Boemare N. 2005; Xenorhabdus . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2B pp. 831–838 Edited by Garrity G. M., Brenner D. J., Krieg N. R., Staley J. T. New York: Springer;
    [Google Scholar]
  4. Boemare N., Akhurst R. J. 1988; Biochemical and physiological characterization of colony form variants in Xenorhabdus spp. (Enterobacteriaceae). J Gen Microbiol 134:751–761
    [Google Scholar]
  5. Edgington S., Buddie A. G., Tymo L., Hunt D. J., Nguyen K. B., France A. I., Merino L. M., Moore D. 2009; Steinernema australe n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile. Nematology 11:699–717 [View Article]
    [Google Scholar]
  6. Felsenstein J. 1988; Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22:521–565 [View Article][PubMed]
    [Google Scholar]
  7. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Koppenhöfer H. S. 2007; Bacterial symbionts of Steinernema and Heterorhabditis . In Entomopathogenic Nematodes: Systematics, Phylogeny and Bacterial Symbionts Nematology Monographs and Perspectives vol. 5 pp. 735–808 Edited by Nguyen K. B., Hunt D. J. Leiden: Brill; [CrossRef]
    [Google Scholar]
  10. Lengyel K., Lang E., Fodor A., Szállás E., Schumann P., Stackebrandt E. 2005; Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov.. Syst Appl Microbiol 28:115–122 [View Article][PubMed]
    [Google Scholar]
  11. Lerat E., Daubin V., Moran N. A. 2003; From gene trees to organismal phylogeny in prokaryotes: the case of the γ-Proteobacteria. PLoS Biol 1:E19 [View Article][PubMed]
    [Google Scholar]
  12. Nguyen K. B., Ginarte C. M., Leite L. G., Santos J. M., Harakava R. 2010; Steinernema brazilense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil. J Invertebr Pathol 103:8–20 [View Article][PubMed]
    [Google Scholar]
  13. Nishimura Y., Hagiwara A., Suzuki T., Yamanaka S. 1994; Xenorhabdus japonicus sp. nov. associated with the nematode Steinernema kushidai . World J Microbiol Biotechnol 10:207–210 [View Article]
    [Google Scholar]
  14. Posada D., Crandall K. A. 1998; MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818 [View Article][PubMed]
    [Google Scholar]
  15. Román J., Figueroa W. 1994; Steinernema puertoricensis n. sp. (Rhabditida: Steinernematidae), a new entomophatogenic nematode from Puerto Rico. J Agric Univ Puerto Rico 78:529–536
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Smith D., Clayton S., Ryan M., Green P. 2001; Preservation methodology. In The UKNCC Biological Resource: Properties, Maintenance and Management pp. 72–121 Edited by Smith D., Ryan M., Day J. Egham, UK: UKNCC;
    [Google Scholar]
  18. Somvanshi V. S., Lang E., Ganguly S., Swiderski J., Saxena A. K., Stackebrandt E. 2006; A novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus indica sp. nov., symbiotically associated with entomopathogenic nematode Steinernema thermophilum Ganguly and Singh, 2000. Syst Appl Microbiol 29:519–525 [View Article][PubMed]
    [Google Scholar]
  19. Swofford D. L. 2003; paup*. Phylogenetic Analysis Using Parsimony (* and Other Methods), version 4.0b10. Sunderland, MA: Sinauer Associates;
  20. Tailliez P., Pagès S., Ginibre N., Boemare N. 2006; New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. Int J Syst Evol Microbiol 56:2805–2818 [View Article][PubMed]
    [Google Scholar]
  21. Tailliez P., Laroui C., Ginibre N., Paule A., Pagès S., Boemare N. 2010; Phylogeny of Photorhabdus and Xenorhabdus based on universally conserved protein-coding sequences and implications for the taxonomy of these two genera. Proposal of new taxa: X. vietnamensis sp. nov., P. luminescens subsp. caribbeanensis subsp. nov., P. luminescens subsp. hainanensis subsp. nov., P. temperata subsp. khanii subsp. nov., P. temperata subsp. tasmaniensis subsp. nov., and the reclassification of P. luminescens subsp. thracensis as P. temperata subsp. thracensis comb. nov.. Int J Syst Evol Microbiol 60:1921–1937 [View Article][PubMed]
    [Google Scholar]
  22. Thomas G., Poinar G. O. Jr 1979; Xenorhabdus gen. nov., a genus of entomopathogenic nematophilic bacteria of the family Enterobacteriaceae. Int J Syst Bacteriol 29:352–360 [View Article]
    [Google Scholar]
  23. Travassos L. 1927; Sobre o genera Oxysomatium . Bol Biol Sao Paulo 5:20–21 (in Portuguese)
    [Google Scholar]
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