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Volume 62, Issue Pt_7

gen. nov., sp. nov. and sp. nov., subdivision 1 from peat and decaying wood Free

Abstract

Two strains of subdivision 1 , a pink-pigmented bacterium KA1 and a colourless isolate WH120, were obtained from acidic peat and wood under decay by the white-rot fungus , respectively. Cells of these isolates were Gram-negative-staining, non-motile, short rods, which were covered by large polysaccharide capsules and occurred singly, in pairs, or in short chains. Strains KA1 and WH120 were strictly aerobic mesophiles that grew between 10 and 33 °C, with an optimum at 22–28 °C. Both isolates developed under acidic conditions, but strain WH120 was more acidophilic (pH growth range 3.5–6.4; optimum, 4.0–4.5) than strain KA1 (pH growth range 3.5–7.3; optimum , 5.0–5.5). The preferred growth substrates were sugars. In addition, the wood-derived isolate WH120 grew on oxalate, lactate and xylan, while the peat-inhabiting acidobacterium strain KA1 utilized galacturonate, glucuronate and pectin. The major fatty acids were iso-C and iso-Cω8; the cells also contained significant amounts of 13,16-dimethyl octacosanedioic acid. The quinone was MK-8. The DNA G+C contents of strains KA1 and WH120 were 54.1 and 51.7 mol%, respectively. Strains KA1 and WH120 displayed 97.8 % 16S rRNA gene sequence similarity to each other. The closest recognized relatives were and (93.4–94.3 % 16S rRNA gene sequence similarity). These species differed from strains KA1 and WH120 by their ability to grow under anoxic conditions, the absence of capsules, presence of cell motility and differing fatty acid composition. Based on these differences, the two new isolates are proposed as representing a novel genus, gen. nov., and two novel species. gen. nov., sp. nov. is the type species for the new genus with strain KA1 ( = DSM 23886 = LMG 25897 = VKM B-2678) as the type strain. The name sp. nov. is proposed for strain WH120 ( = LMG 26244 = VKM B-2677 = NCCB 100371).

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Funding
This study was supported by the:
  • Russian Academy of Sciences
  • RosNauka (Award 02.740.11.0023)
  • Russian Fund of Basic Research (Award 09-04-00004)
  • Institutional Research Concept of the Institute of Microbiology ASCR (Award AV0Z50200510)
© 2012 IUMS
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2012-07-01
2024-04-18
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Acidicapsa borealis gen. nov., sp. nov. and Acidicapsa ligni sp. nov., subdivision 1 Acidobacteria from Sphagnum peat and decaying wood
Int J Syst Evol Microbiol 62, 1512 (2012); https://doi.org/10.1099/ijs.0.034819-0
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