@article{mbs:/content/journal/ijsem/10.1099/ijs.0.034892-0, author = "Hong, Sung Wook and Park, Jung Min and Kim, Soo-Jin and Chung, Kun Sub", title = "Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm (Eisenia fetida L.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_9", pages = "2077-2083", doi = "https://doi.org/10.1099/ijs.0.034892-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.034892-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A swarming and moderately halotolerant bacterium, designated strain A1-2T, was isolated from the intestinal tract of the earthworm Eisenia fetida L. Cells were endospore-forming rods that were facultatively anaerobic, catalase-positive, oxidase-negative and motile by peritrichous flagella. The isolate grew optimally at 30 °C and pH 7.0, and could grow with up to 9 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain A1-2T belonged to the genus Bacillus and exhibited 16S rRNA gene sequence similarities of 96.8, 96.0, 96.0, 96.4 and 96.7 % with Bacillus drentensis LMG 21831T, B. horneckiae PT-45T, B. niacini BAC 1015, B. infantis SMC 4352-1T and B. shackletonii LMG 18435T, respectively. DNA–DNA relatedness values between the isolate and the reference strains were ≤38.3 %. The DNA G+C content of strain A1-2T was 38.5 mol%. The predominant menaquinone was MK-7 and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0 (51.5 %) and anteiso-C15 : 0 (29.6 %) and the cell-wall diamino acid was meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and phenotypic characteristics, it is concluded that strain A1-2T represents a novel species of the genus Bacillus , for which we propose the name Bacillus eiseniae sp. nov. The type strain is A1-2T ( = KCCM 90092T = JCM 16993T).", }