RT Journal Article SR Electronic(1) A1 Hong, Sung Wook A1 Park, Jung Min A1 Kim, Soo-Jin A1 Chung, Kun SubYR 2012 T1 Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm (Eisenia fetida L.) JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_9 SP 2077 OP 2083 DO https://doi.org/10.1099/ijs.0.034892-0 PB Microbiology Society, SN 1466-5034, AB A swarming and moderately halotolerant bacterium, designated strain A1-2T, was isolated from the intestinal tract of the earthworm Eisenia fetida L. Cells were endospore-forming rods that were facultatively anaerobic, catalase-positive, oxidase-negative and motile by peritrichous flagella. The isolate grew optimally at 30 °C and pH 7.0, and could grow with up to 9 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain A1-2T belonged to the genus Bacillus and exhibited 16S rRNA gene sequence similarities of 96.8, 96.0, 96.0, 96.4 and 96.7 % with Bacillus drentensis LMG 21831T, B. horneckiae PT-45T, B. niacini BAC 1015, B. infantis SMC 4352-1T and B. shackletonii LMG 18435T, respectively. DNA–DNA relatedness values between the isolate and the reference strains were ≤38.3 %. The DNA G+C content of strain A1-2T was 38.5 mol%. The predominant menaquinone was MK-7 and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0 (51.5 %) and anteiso-C15 : 0 (29.6 %) and the cell-wall diamino acid was meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and phenotypic characteristics, it is concluded that strain A1-2T represents a novel species of the genus Bacillus , for which we propose the name Bacillus eiseniae sp. nov. The type strain is A1-2T ( = KCCM 90092T = JCM 16993T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.034892-0