1887

Abstract

A polyphasic taxonomic study was carried out on 2C1-b and 2C-21, two strains isolated from sewage flowing into River Geumho in Korea. Cells of the two strains were Gram-negative, non-spore-forming, motile and oval or rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of these two isolates with members of the ; they were most closely related to KCTC 22010, KCTC 12544, KCTC 12583, ATCC 17713 and KACC 11579 showing 16S rRNA gene sequence similarities of 97.4–98.8 % with these strains and shared 100 % similarity with each other. The genomic DNA G+C contents of strains 2C1-b and 2C1-21 were 65.5 and 65.2 mol%, respectively. Phenotypic and chemotaxonomic data [Q-8 as the major ubiquinone; C, summed feature 4 (Cω7 and/or iso-C 2-OH), C cyclo and summed feature 7 (Cω7 and/or ω9 and/or ω12) as major fatty acids] supported the affiliation of strains 2C1-b and 2C-21 to the genus . Based on evidence derived from this polyphasic analysis, it is proposed that strains 2C1-b and 2C1-21 represent a novel species for which the name sp. nov. is proposed; the type strain is 2C1-b ( = KCTC 12768 = JCM 17804).

Funding
This study was supported by the:
  • Advanced Biomass Research Center
  • Global Frontier Program
  • Korean Ministry of Education, Science & Technology
  • CAER (Center for Aquatic Ecosystem Restoration) of Eco-STAR project
  • Ministry of the Environment
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2012-08-01
2024-03-28
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