1887

Abstract

-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376, were found to have identical macrophage infectivity potentiator gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue–white fluorescent pigment. The optimal growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, , ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus for which the name sp. nov. is proposed. The type strain is IMVS-3376 ( = IMVS 3113 = ATCC BAA-2169).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035709-0
2012-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1766.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035709-0&mimeType=html&fmt=ahah

References

  1. Boulanger C. A., Edelstein P. H. 1995; Precision and accuracy of recovery of Legionella pneumophila from seeded tap water by filtration and centrifugation. Appl Environ Microbiol 61:1805–1809[PubMed]
    [Google Scholar]
  2. Brenner D. J. 1987; Classification of the legionellae. Semin Respir Infect 2:190–205[PubMed]
    [Google Scholar]
  3. Cianciotto N. P., Abu Kwaik Y., Edelstein P. H., Fields B. S., Geary D. F., Harrison T. G., Joseph C. A., Ratcliff R. M., Stout J. E. other authors 2006 Legionella: state of the art 30 years after its recognition Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Edelstein P. H. 2004; Detection of Legionella antigen by direct immunofluorescence. In Clinical Microbiology Procedures Handbook pp. 11.3.1–11.3.7 Edited by Isenberg H. D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Edelstein P. H., Cianciotto N. P. 2010; Legionella . In Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases, 7th edn. pp. 2969–2984 Edited by Mandell G. L. J. E. Bennett & R. Dolln. Philadelphia: Elsevier;
    [Google Scholar]
  6. Edelstein P. H., Edelstein M. A. C. 1991; Comparison of different agars used in the formulation of buffered charcoal yeast extract medium. J Clin Microbiol 29:190–191[PubMed]
    [Google Scholar]
  7. Edelstein P. H., Edelstein M. A. C. 1993; Comparison of three buffers used in the formulation of buffered charcoal yeast extract medium. J Clin Microbiol 31:3329–3330[PubMed]
    [Google Scholar]
  8. Edelstein P. H., Brenner D. J., Moss C. W., Steigerwalt A. G., Francis E. M., George W. L. 1982; Legionella wadsworthii species nova: a cause of human pneumonia. Ann Intern Med 97:809–813[PubMed] [CrossRef]
    [Google Scholar]
  9. Edelstein P. H., Beer K. B., DeBoynton E. D. 1987; Influence of growth temperature on virulence of Legionella pneumophila . Infect Immun 55:2701–2705[PubMed]
    [Google Scholar]
  10. Higa F., Edelstein P. H. 2001; Potential virulence role of the Legionella pneumophila ptsP ortholog. Infect Immun 69:4782–4789 [View Article][PubMed]
    [Google Scholar]
  11. Huson D. H., Bryant D. 2006; Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267 [View Article][PubMed]
    [Google Scholar]
  12. Ko K. S., Lee H. K., Park M. Y., Lee K. H., Yun Y. J., Woo S. Y., Miyamoto H., Kook Y. H. 2002; Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species. J Clin Microbiol 40:2653–2658 [View Article][PubMed]
    [Google Scholar]
  13. Kodaka H., Armfield A. Y., Lombard G. L., Dowell V. R. Jr 1982; Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16:948–952[PubMed]
    [Google Scholar]
  14. Lück P. C., Jacobs E., Röske I., Schröter-Bobsin U., Dumke R., Gronow S. 2010; Legionella dresdenensis sp. nov. isolated from river water. Int J Syst Evol Microbiol 60:2557–2562 [View Article][PubMed]
    [Google Scholar]
  15. Miller M. A., Pfeiffer W., Schwartz T. 2010; Creating the CIPRES science gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop pp. 1–8 New Orleans, LA: Gateway Computing Environments Workshop;
    [Google Scholar]
  16. Posada D., Crandall K. A. 1998; modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [View Article][PubMed]
    [Google Scholar]
  17. Ratcliff R. M., Lanser J. A., Manning P. A., Heuzenroeder M. W. 1998; Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 36:1560–1567[PubMed]
    [Google Scholar]
  18. Rubin C. J., Thollesson M., Kirsebom L. A., Herrmann B. 2005; Phylogenetic relationships and species differentiation of 39 Legionella species by sequence determination of the RNase P RNA gene rnpB. Int J Syst Evol Microbiol 55:2039–2049 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035709-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035709-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error