1887

Abstract

A Gram-stain-positive, oxidase-negative, starch-hydrolysing, actinobacterium (strain JC82) was isolated from a soda lake in Lonar, India. Based on 16S rRNA gene sequence similarity studies, strain JC82 belonged to the genus and was most closely related to 1A-C (96.8 %) and other members of the genus (<96.5 %). The DNA G+C content of strain JC82 was 73.4 mol%. The cell-wall amino acids were alanine, glutamic acid and lysine with peptidoglycan type A4α. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid (L1) and an unidentified glycolipid (GL3). The predominant isoprenoid quinone was menaquinone MK-8(H). Anteiso-C was the predominant fatty acid and significant proportions of iso-C, C, C, iso-C and iso-C were also detected. Strain JC82 produced thermostable alkaline amylase. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain JC82 from all other members of the genus . Based on these data, strain JC82 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC82 ( = KCTC 19802 = NBRC 107612).

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2012-10-01
2024-03-29
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References

  1. Altenburger P., Kämpfer P., Schumann P., Vybiral D., Lubitz W., Busse H.-J. 2002; Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52:875–881 [View Article][PubMed]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  3. Anil Kumar P., Srinivas T. N. R., Madhu S., Manorama R., Shivaji S. 2010a; Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol 60:721–726 [View Article][PubMed]
    [Google Scholar]
  4. Anil Kumar P., Srinivas T. N. R., Pavan Kumar P., Madhu S., Shivaji S. 2010b; Nitritalea halalkaliphila gen. nov., sp. nov., an alkaliphilic bacterium of the family ‘Cyclobacteriaceae’, phylum Bacteroidetes . Int J Syst Evol Microbiol 60:2320–2325 [View Article][PubMed]
    [Google Scholar]
  5. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  6. Cappuccino J. G., Sherman N. 1998 Microbiology: a Laboratory Manual, 5th edn. California: Benjamin/Cummings Science Publishing;
    [Google Scholar]
  7. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  8. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 30:435–448 [View Article]
    [Google Scholar]
  9. Hiraishi A., Hoshino Y., Kitamura H. 1984; Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 30:197–210 [View Article]
    [Google Scholar]
  10. Imhoff J. F., Pfennig N. 2001; Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b . Int J Syst Evol Microbiol 51:105–110[PubMed]
    [Google Scholar]
  11. Imhoff J. F., Süling J., Petri R. 1998; Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48:1129–1143 [View Article][PubMed]
    [Google Scholar]
  12. Joshi A. A., Kanekar P. P., Kelkar A. S., Shouche Y. S., Vani A. A., Borgave S. B., Sarnaik S. S. 2008; Cultivable bacterial diversity of alkaline Lonar Lake, India. Microb Ecol 55:163–172 [View Article][PubMed]
    [Google Scholar]
  13. Kates M. 1972 Techniques of Lipidology New York: Elsevier;
    [Google Scholar]
  14. Kates M. 1986 Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids Amsterdam: Elsevier;
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  16. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [View Article][PubMed]
    [Google Scholar]
  17. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  19. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. 2000; A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30:178–182 [View Article][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  21. Nelson N. 1944; A photometric adaptation of the somogyi method for the determination of glucose. J Biol Chem 153:375–380
    [Google Scholar]
  22. Oren A., Duker S., Ritter S. 1996; The polar lipid composition of Walsby’s square bacterium. FEMS Microbiol Lett 138:135–140 [View Article]
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74:5463–5467 [View Article][PubMed]
    [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
    [Google Scholar]
  25. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  26. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  28. Tang S.-K., Wang Y., Lou K., Mao P.-H., Xu L.-H., Jiang C.-L., Kim C.-J., Li W.-J. 2009a; Kocuria halotolerans sp. nov., an actinobacterium isolated from a saline soil in China. Int J Syst Evol Microbiol 59:1316–1320 [View Article][PubMed]
    [Google Scholar]
  29. Tang S.-K., Wang Y., Chen Y., Lou K., Cao L.-L., Xu L.-H., Li W.-J. 2009b; Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2031 [View Article][PubMed]
    [Google Scholar]
  30. Tang S.-K., Wang Y., Lee J.-C., Lou K., Park D.-J., Kim C.-J., Li W.-J. 2010; Georgenia halophila sp. nov., a halophilic actinobacterium isolated from a salt lake. Int J Syst Evol Microbiol 60:1317–1421 [View Article][PubMed]
    [Google Scholar]
  31. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  32. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  33. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  34. Wani A. A., Surakasi V. P., Siddharth J., Raghavan R. G., Patole M. S., Ranade D., Shouche Y. S. 2006; Molecular analyses of microbial diversity associated with the Lonar soda lake in India: an impact crater in a basalt area. Res Microbiol 157:928–937 [View Article][PubMed]
    [Google Scholar]
  35. Woo S. G., Cui Y., Kang M. S., Jin L., Kim K. K., Lee S.-T., Lee M., Park J. 2012; Georgenia daeguensis sp. nov., isolated from 4-chlorophenol enrichment culture. Int J Syst Evol Microbiol. 62:1703–1709 [View Article][PubMed]
    [Google Scholar]
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