1887

Abstract

A novel Gram-staining-positive, rod-shaped, motile, strictly aerobic, endospore-forming bacterium, designated WCC 4585, was isolated from a pharmaceutical production line. The organism grew optimally at 30 °C, at pH 8 and in the presence of 0.5 % (w/v) NaCl. Oval endospores were formed subterminally and terminally in swollen sporangia. The cell-wall diamino acid was -diaminopimelic acid (type A1γ) and the genomic DNA G+C content was 38.7 mol%. The major menaquinone was MK-7. The cellular fatty acid profile contained major amounts of iso-C, anteiso-C and anteiso-C, and the cellular phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. The isolate was most closely related to H2, SMC 4352-1, NCIMB 9366, ATCC 4513 and DSM 23495 with which it shared less than 98.0 % 16S rRNA gene sequence similarity. DNA–DNA relatedness values between strain WCC 4585 and five type strains of related species were ≤27 % and sequence similarity values based on sequences were ≤88.7 %. On the basis of the characteristics presented, strain WCC 4585 is proposed to represent a novel species, sp. nov. The type strain is WCC 4585( = DSM 23668 = CCUG 59876 = LMG 25856).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.036277-0
2013-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/867.html?itemId=/content/journal/ijsem/10.1099/ijs.0.036277-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  3. European Commission ( 2008 ). EudraLex. The Rules Governing Medicinal Products in the European Union EU. Guidelines to Good Manufacturing Practice - Medicinal Products for Human and Veterinary Use. Annex 1: Manufacture of Sterile Medicinal Products. http://ec.europa.eu/health/files/eudralex/vol-4/2008_11_25_gmp-an1_en.pdf
  4. Goh S. H., Potter S., Wood J. O., Hemmingsen S. M., Reynolds R. P., Chow A. W. ( 1996 ). HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. . J Clin Microbiol 34, 818823.[PubMed]
    [Google Scholar]
  5. Gouy M., Guindon S., Gascuel O. ( 2010 ). SeaView version 4: A multiplatform graphical user interface for sequence alignment and phylogenetic tree building. . Mol Biol Evol 27, 221224. [View Article] [PubMed]
    [Google Scholar]
  6. Harrigan W. F., McCance M. E. ( 1976 ). Laboratory Methods in Foods and Dairy Microbiology. London:: Academic Press;.
    [Google Scholar]
  7. Logan N. A., De Vos P. ( 2009 ). Genus I. Bacillus . . In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 21128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  8. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  9. Pearson W. R., Lipman D. J. ( 1988 ). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85, 24442448. [View Article] [PubMed]
    [Google Scholar]
  10. Ryu E. ( 1940 ). A simple method of differentiation between Gram-positive and Gram-negative organisms without staining. . Kitasato Arch Exp Med 17, 5863.
    [Google Scholar]
  11. Schmidt V. S., Mayr R., Wenning M., Glöckner J., Busse H.-J., Scherer S. ( 2009 ). Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese. . Int J Syst Evol Microbiol 59, 24372443. [View Article] [PubMed]
    [Google Scholar]
  12. Seiler H., Schmidt V., Wenning M., Scherer S. ( 2012 ). Bacillus kochii sp. nov., isolated from foods and a pharmaceutical manufacturing site. . Int J Syst Evol Microbiol 62, 10921097. [View Article]
    [Google Scholar]
  13. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). MEGA4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  14. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  15. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60, 249266. [View Article] [PubMed]
    [Google Scholar]
  16. Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K. ( 2010 ). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. . Int J Syst Evol Microbiol 60, 10311037. [View Article] [PubMed]
    [Google Scholar]
  17. Van de Peer Y., De Wachter R. ( 1997 ). Construction of evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites. . Comput Appl Biosci 13, 227230.[PubMed]
    [Google Scholar]
  18. Venkateswaran K., Kempf M., Chen F., Satomi M., Nicholson W., Kern R. ( 2003 ). Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are γ-radiation resistant. . Int J Syst Evol Microbiol 53, 165172. [View Article] [PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  20. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [View Article] [PubMed]
    [Google Scholar]
  21. Zhang Z., Schwartz S., Wagner L., Miller W. ( 2000 ). A greedy algorithm for aligning DNA sequences. . J Comput Biol 7, 203214. [View Article] [PubMed]
    [Google Scholar]
  22. Zhang L., Wang Y., Dai J., Tang Y., Yang Q., Luo X., Fang C. ( 2009 ). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. . Int J Syst Evol Microbiol 59, 17871792. [View Article] [PubMed]
    [Google Scholar]
  23. Zhang J., Wang J., Fang C., Song F., Xin Y., Qu L., Ding K. ( 2010 ). Bacillus oceanisediminis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60, 29242929. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.036277-0
Loading
/content/journal/ijsem/10.1099/ijs.0.036277-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error