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Volume 63, Issue Pt_1

gen. nov., sp. nov., an actinobacterium of the family Free

Abstract

Three novel actinobacteria, strains 39, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39 represented a novel lineage within the family and was most closely related to members of the genera (96.9 % 16S rRNA gene sequence similarity), (95.7 %), (94.4–95.3 %), (94.6 %), (94.3 %), (94.2 %) and (93.1 %). Cells were irregular cocci and short rods. The peptidoglycan type was A4α with an -Lys–-Ser–-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was -acetyl. The major cellular fatty acids were anteiso-C (41.97 %), anteiso-Cω9 (32.16 %) and iso-C (7.68 %). The DNA G+C content of strain 39 was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family , a novel genus and species, gen. nov., sp. nov., is proposed; the type strain of the type species is 39 ( = CGMCC 4.6864  = DSM 24617).

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Funding
This study was supported by the:
  • Malaysia Antarctic Research Program
  • Academy of Sciences Malaysia
  • Universiti Putra Malaysia Research Universiti Grant Scheme (Award 05-01-11-1219RU)
© 2013 IUMS
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2013-01-01
2024-04-18
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Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae
Int J Syst Evol Microbiol 63, 241 (2013); https://doi.org/10.1099/ijs.0.038232-0
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