@article{mbs:/content/journal/ijsem/10.1099/ijs.0.038646-0, author = "Alegado, Rosanna A. and Grabenstatter, Jonathan D. and Zuzow, Richard and Morris, Andrea and Huang, Sherri Y. and Summons, Roger E. and King, Nicole", title = "Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_1", pages = "163-168", doi = "https://doi.org/10.1099/ijs.0.038646-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.038646-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1T, was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1T grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1T contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C15 : 0 (29.4 %), iso-C17 : 1ω9c (18.5 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 11.3 %). The major polar lipids detected in strain PR1T were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1T fell within the cluster comprising the genus Algoriphagus and was most closely related to Algoriphagus halophilus JC 2051T (95.4 % sequence similarity) and Algoriphagus lutimaris S1-3T (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1T and the type strains of other species of the genus Algoriphagus were in the range 91–95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1T demonstrated that this strain was distinct from other members of the genus Algoriphagus , including its closest relative, A. halophilus . Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1T should be placed in the genus Algoriphagus as a representative of a novel species, for which the name Algoriphagus machipongonensis sp. nov. is proposed. The type strain is PR1T ( = ATCC BAA-2233T  = DSM 24695T).", }