Gordonia alkaliphila sp. nov., an actinomycete isolated from tidal flat sediment
Cha, Ju-Hee and Cha, Chang-Jun,, 63, 327-331 (2013),
doi = https://doi.org/10.1099/ijs.0.039289-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-positive, non-motile, aerobic actinobacterium, designated strain CJ10T, was isolated from tidal flat sediment from the Yellow Sea in South Korea. Strain CJ10T grew on tryptic soy agar in the presence of 0–4 % (w/v) NaCl (optimum growth in the absence of NaCl) and at pH 6–11 (optimum pH 9). On the basis of 16S rRNA gene sequence analysis, strain CJ10T belonged to the genus Gordonia and showed the highest sequence similarity to Gordonia hirsuta DSM 44140T (97.9 %) and Gordonia hydrophobica DSM 44015T (97.6 %). DNA–DNA relatedness levels of strain CJ10T were 47.4 % (CJ10T as probe) and 42.2 % ( G. hirsuta DSM 44140T as probe) to G. hirsuta DSM 44140T and 8.6 % (CJ10T as probe) and 9.3 % ( G. hydrophobica DSM 44015T as probe) to G. hydrophobica DSM 44015T. The major isoprenoid quinone was MK-9(H2). The polar lipid profile of strain CJ10T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant cellular fatty acids were C18 : 1ω9c (38.0 %), C16 : 0 (30.1 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 17.4 %). The DNA G+C content was 67.7 mol%. Therefore, the results from our polyphasic taxonomic study suggest that strain CJ10T represents a novel species in the genus Gordonia , for which the name Gordonia alkaliphila sp. nov. is proposed; the type strain is CJ10T ( = KACC 16561T  = JCM 18077T).,
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