Brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant
Kim, Jinsoo and Srinivasan, Sathiyaraj and You, Taek and Bang, John J. and Park, Sujeong and Lee, Sang-Seob,, 63, 1111-1118 (2013),
doi = https://doi.org/10.1099/ijs.0.039305-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated A1T, was isolated from sludge of a wastewater treatment plant. Strain A1T showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. Strain A1T grew with 0–11 % (w/v) NaCl, at 20–42 °C, but not <15 or >45 °C and at pH 6–10 (optimum pH 8.0–9.0). The isolate was catalase-positive and oxidase-negative. The DNA G+C content was 70.7 mol%. A comparative analysis of 16S rRNA gene sequences revealed that strain A1T formed a distinct phyletic lineage in the genus Brevibacterium and showed high sequence similarity with Brevibacterium casei NCDO 2048T (96.9 %), Brevibacterium celere KMM 3637T (96.9 %) and Brevibacterium sanguinis CF63T (96.4 %). DNA–DNA hybridization revealed <43 % DNA–DNA relatedness between the isolate and its closest phylogenetic relatives. The affiliation of strain A1T with the genus Brevibacterium was supported by the chemotaxonomic data: predominant quinone menaquinone MK-7(H2); polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; whole-cell sugars galactose, xylose and ribose; absence of mycolic acids; and major fatty acids iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The results of physiological and biochemical tests allowed phenotypic differentiation of strain A1T from members of the genus Brevibacterium . On the basis of the results in this study, a novel species, Brevibacterium ammoniilyticum sp. nov., is proposed. The type strain is A1T ( = KEMC 41-098T  = JCM 17537T  = KACC 15558T).,
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