RT Journal Article SR Electronic(1) A1 Zhang, Jun A1 Wang, Yueqiang A1 Zhou, Shungui A1 Wu, Chunyuan A1 He, Jian A1 Li, FangbaiYR 2013 T1 Comamonas guangdongensis sp. nov., isolated from subterranean forest sediment, and emended description of the genus Comamonas JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_3 SP 809 OP 814 DO https://doi.org/10.1099/ijs.0.040188-0 PB Microbiology Society, SN 1466-5034, AB A facultatively anaerobic bacterium, strain CY01T, isolated from subterranean forest sediment collected from Guangdong Province, China, was investigated using a polyphasic taxonomic approach. The cells were short rods, Gram-negative, non-sporulating and motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CY01T showed highest sequence similarities to Comamonas thiooxydans S23T (98.0 %), Comamonas testosteroni JCM 5832T (97.9 %), Comamonas koreensis KCTC 12005T (97.7 %) and Comamonas odontotermitis LMG 23579T (97.0 %). The major respiratory quinone was ubiquinone-8. The major cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain CY01T was clearly distinguishable from all recognized species of the genus Comamonas and should be classified as a representative of a novel species of the genus, for which the name Comamonas guangdongensis sp. nov. is proposed. The type strain is CY01T ( = CCTCC AB 2011133T = KACC 16241T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.040188-0