Galenea microaerophila gen. nov., sp. nov., a mesophilic, microaerophilic, chemosynthetic, thiosulfate-oxidizing bacterium isolated from a shallow-water hydrothermal vent
Giovannelli, Donato and Grosche, Ashley and Starovoytov, Valentin and Yakimov, Michail and Manini, Elena and Vetriani, Costantino,, 62, 3060-3066 (2012),
doi = https://doi.org/10.1099/ijs.0.040808-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A mesophilic, strictly microaerophilic, chemosynthetic bacterium, designated strain P2DT, was isolated from the sediment of an active shallow-water hydrothermal vent in Paleochori Bay, on the Greek island of Milos. The cells were Gram-staining-negative rods that measured approximately 0.8–1.3 µm in length and 0.4–0.5 µm in width. Strain P2DT grew at 20–50 °C (optimum 35 °C), with 1.0–5.0 % (w/v) NaCl (optimum 3.0 %), and at pH 4.5–8.0 (optimum pH 5.5). The generation time under optimal conditions was 1.1 h. Growth occurred under chemolithoautotrophic conditions with S 2 O 3 2 - and CO2 as the energy and carbon sources, respectively. Oxygen (5 %) was used as sole terminal electron acceptor. No growth was observed in the presence of acetate, formate, lactate, tryptone or peptone. Chemolithoheterotrophic growth occurred when d-glucose or sucrose were present as carbon sources. None of the organic compounds tested was used as an electron donor. The genomic DNA G+C content of the novel strain was 44.9 mol%. In a phylogenetic analysis based on 16S rRNA gene sequences, strain P2DT was found to be most closely related to Thiomicrospira psychrophila DSM 13453T (92.8% sequence similarity). Based on the phylogenetic, physiological and chemotaxonomic evidence, strain P2DT represents a novel species of a new genus within the class Gammaproteobacteria of the family Piscirickettsiaceae , for which the name Galenea microaerophila gen. nov., sp. nov. is proposed. The type strain of the type species is P2DT ( = DSM 24963T = JCM 17795T).,
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