@article{mbs:/content/journal/ijsem/10.1099/ijs.0.041178-0, author = "Chen, Wen-Ming and Huang, Wei-Cheng and Young, Chiu-Chung and Sheu, Shih-Yi", title = "Flavobacterium tilapiae sp. nov., isolated from a freshwater pond, and emended descriptions of Flavobacterium defluvii and Flavobacterium johnsoniae", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_3", pages = "827-834", doi = "https://doi.org/10.1099/ijs.0.041178-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.041178-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A bacterial strain, designated Ruye-71T, was isolated from a freshwater tilapiine cichlid fish culture pond in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain Ruye-71T were Gram-stain-negative, strictly aerobic, yellow-pigmented rods that were motile by gliding. Growth occurred at 10–30 °C (optimum, 15–30 °C), at pH 7.0–8.0 (optimum, pH 8.0) and with 0–2 % NaCl (optimum, 0–1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-71T belonged to the genus Flavobacterium and was most closely related to Flavobacterium defluvii EMB117T, with a 16S rRNA gene sequence similarity of 97.7 %. Strain Ruye-71T contained iso-C15 : 0, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0, C16 : 0 3-OH, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several unidentified polar lipids. The DNA G+C content of the genomic DNA of strain Ruye-71T was 39.2 mol%. The mean level of DNA–DNA relatedness between strain Ruye-71T and Flavobacterium defluvii DSM 17963T was 39.9±1.2 %. On the basis of phylogenetic inference and phenotypic data, strain Ruye-71T should be classified as representing a novel species, for which the name Flavobacterium tilapiae sp. nov. is proposed. The type strain is Ruye-71T ( = BCRC 80262T = KCTC 23312T). Emended descriptions of Flavobacterium defluvii and Flavobacterium johnsoniae are also proposed.", }