1887

Abstract

Five strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. Phylogenetic analysis of full-length 16S rRNA gene sequences showed that these strains represented a novel group within the family , and the most closely related species was . and the novel taxon are deeply branched from the genus , with sequence identities to the type strain of the type species of , , of 82.2 and 85.6 %, respectively. The novel genus gen. nov. is proposed to accommodate the novel species gen. nov., sp. nov. and the previously named Moore 1994 as gen. nov., comb. nov. The type species is . The type strain of is 81/4-12 ( = DSM 22548  = CCUG 58091) and the type strain of is ATCC 51259  = CCUG 34292  = CIP 104476  = NCTC 13073. is weakly to moderately saccharolytic and produces moderate amounts of acetic acid and major amounts of succinic acid as end products of fermentation. Strains are sensitive to 20 % bile and hydrolyse gelatin. The principal cellular long-chain fatty acids are anteiso-C, iso-C, C, iso-C and iso-C 3-OH. The G+C content of the DNA of the type strain is 47 mol%.

Funding
This study was supported by the:
  • Guy’s and St Thomas’ Charity (Award R050724)
  • National Institute for Dental Research (Award DE016937)
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2013-04-01
2024-04-19
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Chen T., Yu W. H., Izard J., Baranova O. V., Lakshmanan A., Dewhirst F. E. ( 2010 ). The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information. . Database (Oxford) 2010, baq013. [View Article] [PubMed]
    [Google Scholar]
  3. Dewhirst F. E., Chen T., Izard J., Paster B. J., Tanner A. C., Yu W. H., Lakshmanan A., Wade W. G. ( 2010 ). The human oral microbiome. . J Bacteriol 192, 50025017. [View Article] [PubMed]
    [Google Scholar]
  4. Downes J., Sutcliffe I., Tanner A. C., Wade W. G. ( 2005 ). Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. . Int J Syst Evol Microbiol 55, 15511555. [View Article] [PubMed]
    [Google Scholar]
  5. Hall T. ( 2004 ). BioEdit: Biological Sequence Alignment Editor for Win95/98/NT/2K/XP. Carlsbad, CA:: Ibis Biosciences;.
    [Google Scholar]
  6. Holdeman L. V., Cato E. P., Moore W. E. C. ( 1977 ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  7. Jousimies-Somer H. R., Summanen P., Citron D. M., Baron E.-J., Wexler H. M., Finegold S. M. ( 2002 ). Wadsworth-KTL Anaerobic Bacteriology Manual. Belmont, CA:: Star Publishing Company;.
    [Google Scholar]
  8. Kononen E., Wade W. G., Citron D. M. ( 2010 ). Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic Gram-negative rods. . In Manual of Clinical Microbiology, , 10th edn., pp. 858880. Edited by Versalovic J., Carroll K. C., Funke G., Jorgensen J. H., Landry M. L., Warnock D. W. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358361. [View Article]
    [Google Scholar]
  10. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584586.[PubMed]
    [Google Scholar]
  11. Moore L. V., Johnson J. L., Moore W. E. ( 1994 ). Descriptions of Prevotella tannerae sp. nov. and Prevotella enoeca sp. nov. from the human gingival crevice and emendation of the description of Prevotella zoogleoformans . . Int J Syst Bacteriol 44, 599602. [View Article] [PubMed]
    [Google Scholar]
  12. Morotomi M., Nagai F., Sakon H., Tanaka R. ( 2009 ). Paraprevotella clara gen. nov., sp. nov. and Paraprevotella xylaniphila sp. nov., members of the family ‘Prevotellaceae’ isolated from human faeces. . Int J Syst Evol Microbiol 59, 18951900. [View Article] [PubMed]
    [Google Scholar]
  13. Sakamoto M., Tanner A. C. R., Benno Y. ( 2010 ). Genus VII. Tannerella . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 7882. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. . New York:: Springer;.
    [Google Scholar]
  14. Shah H. N., Chattaway M. A., Rajakurana L., Gharbia S. E. ( 2010 ). Genus I. Prevotella . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 86102. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. . New York:: Springer;.
    [Google Scholar]
  15. Wade W. G., Downes J., Dymock D., Hiom S. J., Weightman A. J., Dewhirst F. E., Paster B. J., Tzellas N., Coleman B. ( 1999 ). The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov.. Int J Syst Bacteriol 49, 595600. [View Article] [PubMed]
    [Google Scholar]
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