1887

Abstract

A novel bacterium, designated F081-2, isolated from seawater from Damupo beach in Pohang, Korea, was investigated using a polyphasic taxonomic approach. Cells were yellow-pigmented, strictly aerobic, motile by gliding, Gram-negative-staining and rod-shaped. The temperature, pH and NaCl ranges for growth were 4–35 °C, pH 5.5–9.5 and 1.0–5.0 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F081-2 belonged to a distinct lineage in the genus of the family sharing 93.7–98.1 % similarity with recognized members of the genus. Low levels of DNA–DNA relatedness values were found between strain F081-2 and KCTC 12219 (61.1 %), KCTC 12221 (47.0 %), KCTC 22026 (39.3 %), CCUG 56098 (34.3 %) and JCM 17633 (33.4 %). The major cellular fatty acids were iso-C (25.3 %), iso-C G (14.6 %), iso-C 3-OH (9.3 %), anteiso-C (7.8 %) and iso-C 3-OH (7.6 %). The polar lipid profile was composed of phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid, one unidentified aminophospholipid and six unidentified lipids. The major respiratory quinone was menaquinone-6 and the DNA G+C content of the strain was 32.3 mol%. On the basis of phenotypic, phylogenetic and genotypic data, strain F081-2 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is F081-2 ( = KCTC 23552 = JCM 17633).

Funding
This study was supported by the:
  • 21C Frontier Microbial Genomics and Applications Centre Program, Ministry of Education, Science & Technology, Korea
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2013-01-01
2024-04-18
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References

  1. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . . In The Prokaryotes, vol. 1, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. . Berlin:: Springer;.
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y. ( 2006 ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes. A Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [View Article] [PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y. Y., Kim M. J., Kim S. I., Kim B. K., Lim Y. W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [View Article] [PubMed]
    [Google Scholar]
  5. CLSI (2003). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 8th edn, M2–A8. Wayne, PA: Clinical Laboratory Standards Institute.
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  9. Hall T. ( 2007 ). BioEdit. Biological sequence alignment editor for Win95/98/NT/2K/XP. Carlsbad, CA:: Ibis Biosciences;.
    [Google Scholar]
  10. Ivanova E. P., Christen R., Gorshkova N. M., Zhukova N. V., Kurilenko V. V., Crawford R. J., Mikhailov V. V. ( 2010 ). Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella . . Int J Syst Evol Microbiol 60, 15771580. [View Article] [PubMed]
    [Google Scholar]
  11. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. ( 2004 ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54, 9397. [View Article] [PubMed]
    [Google Scholar]
  12. Kim S. B., Nedashkovskaya O. I. ( 2010 ). Winogradskyella pacifica sp. nov., a marine bacterium of the family Flavobacteriaceae . . Int J Syst Evol Microbiol 60, 19481951. [View Article] [PubMed]
    [Google Scholar]
  13. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  15. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299306. [View Article] [PubMed]
    [Google Scholar]
  16. Lányi B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  17. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Dobretsov S., Lau K. W. K., Wu M., Wong P. K., Pawlik J. R., Qian P.-Y. ( 2005 ). Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. . Int J Syst Evol Microbiol 55, 15891592. [View Article] [PubMed]
    [Google Scholar]
  18. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109118. [View Article] [PubMed]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Han S. K., Snauwaert C., Vancanneyt M., Swings J., Kim K. O., Lysenko A. M., Rohde M. & other authors ( 2005 ). Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae . . Int J Syst Evol Microbiol 55, 4955. [View Article] [PubMed]
    [Google Scholar]
  21. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Zhukova N. V. ( 2009 ). Winogradskyella echinorum sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . . Int J Syst Evol Microbiol 59, 14651468. [View Article] [PubMed]
    [Google Scholar]
  22. Pinhassi J., Nedashkovskaya O. I., Hagström Å., Vancanneyt M. ( 2009 ). Winogradskyella rapida sp. nov., isolated from protein-enriched seawater. . Int J Syst Evol Microbiol 59, 21802184. [View Article] [PubMed]
    [Google Scholar]
  23. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. ( 2009 ). Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. . Int J Syst Evol Microbiol 59, 14431446. [View Article] [PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  25. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  26. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal _ x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  28. Tindall B. J., Sikorski J., Simbert R. A., Kreig N. R. ( 2007 ). Phenotypic characterization and principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Tourova T. P., Antonov A. S. ( 1988 ). Identification of microorganisms by rapid DNA-DNA hybridization. . Methods in Microbiol 19, 333355. [View Article]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  31. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  32. Yoon B.-J., Byun H.-D., Kim J.-Y., Lee D.-H., Kahng H.-Y., Oh D.-C. ( 2011 ). Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella . . Int J Syst Evol Microbiol 61, 15391543. [View Article] [PubMed]
    [Google Scholar]
  33. ZoBell C. E. ( 1941 ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. . J Mar Res 4, 4275.
    [Google Scholar]
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