Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana
Glaeser, Stefanie P. and Galatis, Harald and Martin, Karin and Kämpfer, Peter,, 63, 521-525 (2013),
doi = https://doi.org/10.1099/ijs.0.041392-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain E103T, was isolated from the skin of the medical leech Hirudo verbana. 16S rRNA gene sequence analysis showed that the isolate was closely related to species of the genus Castellaniella . Castellaniella ginsengisoli DCY36T was shown to be the most closely related (98.4 % 16S rRNA gene sequence similarity), followed by Castellaniella denitrificans NKNTAUT and Castellaniella daejeonensis MJ06T (both 97.8 %), then Castellaniella caeni Ho-11T (97.5 %). Chemotaxonomic data (major ubiquinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine; predominant polyamine, putrescine with a moderate amount of 2-hydroxyputrescine; and major fatty acids, C17 : 0 cyclo, C16 : 0 and summed feature 4 comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) supported the affiliation of the isolate to the genus Castellaniella . DNA–DNA hybridization values with the type strains of all species of the genus Castellaniella were 23 % (reciprocal, 18 %) with C. ginsengisoli KCTC 22398T, 20 % (26 %) with C. daejeonensis KCTC 22454T, 11 % (58 %) with C. denitrificans DSM 11046T and 13 % (12 %) with C. caeni KCTC 12197T . Phenotypic differentiation of strain E103T from its closest neighbours was possible. Strain E103T therefore represents a novel species of the genus Castellaniella , for which the name Castellaniella hirudinis sp. nov. is proposed, with the type strain E103T ( = CCUG 62394T = LMG 26910T).,
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