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Abstract

A taxonomic study was carried out on a novel bacterium, designated strain 3-C-1, which was isolated from a crude-oil-degrading consortium produced by enrichment of a sample of surface seawater collected near Xiamen Island in China. Cells of the novel strain were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped, motile and amphitrichous. Growth was observed with 0–12 % (w/v) NaCl and at temperatures of 10–42 °C. The novel strain was incapable of nitrate reduction. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-C-1 belonged to the genus and was most closely related to CEE_131 (with sequence similarities of 96.5–96.8 %). The gene sequence of strain 3-C-1 was also similar to that of CEE_131 (89.4 % sequence similarity). The results of PCR-based comparison of repetitive extragenic palindromic elements (Rep-PCR fingerprinting) allowed strain 3-C-1 to be distinguished from CEE_131. The principal fatty acids of the novel strain were C, C 3-OH, summed feature 3 (Cω6 and/or Cω7) and summed feature 8 (Cω6 and/or Cω7). The G+C content of the novel strain’s genomic DNA was 61.3 mol%. The combined genotypic and phenotypic data indicate that strain 3-C-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3-C-1 ( = CCTCC AB 209060  = LMG 25226  = MCCC 1A01354).

Funding
This study was supported by the:
  • State Oceanic Administration, China (Award 201005032)
  • State Oceanic Administration, China (Award 2010137)
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2011-9)
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2013-03-01
2024-03-29
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ( 1995 ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Lai Q., Yuan J., Wang B., Sun F., Qiao N., Zheng T., Shao Z. ( 2009 ). Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. . Int J Syst Evol Microbiol 59, 15791582. [View Article] [PubMed]
    [Google Scholar]
  3. Liu C., Shao Z. ( 2005 ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55, 11811186. [View Article] [PubMed]
    [Google Scholar]
  4. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. . J Chromatogr A 479, 297306. [View Article] [PubMed]
    [Google Scholar]
  5. Rodríguez-Blanco A., Vetion G., Escande M. L., Delille D., Ghiglione J. F. ( 2010 ). Gallaecimonas pentaromativorans gen. nov., sp. nov., a bacterium carrying 16S rRNA gene heterogeneity and able to degrade high-molecular-mass polycyclic aromatic hydrocarbons. . Int J Syst Evol Microbiol 60, 504509. [View Article] [PubMed]
    [Google Scholar]
  6. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9, 945967.
    [Google Scholar]
  7. Rzhetsky A., Nei M. ( 1993 ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10, 10731095.[PubMed]
    [Google Scholar]
  8. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  9. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  10. Stackebrandt E., Goebel B. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  11. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  12. Versalovic J., Koeuth T., Lupski J. R. ( 1991 ). Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. . Nucleic Acids Res 19, 68236831. [View Article] [PubMed]
    [Google Scholar]
  13. Wang W., Wang L., Shao Z. ( 2010 ). Diversity and abundance of oil-degrading bacteria and alkane hydroxylase (alkB) genes in the subtropical seawater of Xiamen Island. . Microb Ecol 60, 429439. [View Article] [PubMed]
    [Google Scholar]
  14. Wang L., Lai Q., Fu Y., Chen H., Wang W., Wang J., Sun F., Shao Z. ( 2011 ). Idiomarina xiamenensis sp. nov., isolated from surface seawater, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov.. Int J Syst Evol Microbiol 61, 969973. [View Article] [PubMed]
    [Google Scholar]
  15. Yamamoto S., Kasai H., Arnold D. L., Jackson R. W., Vivian A., Harayama S. ( 2000 ). Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. . Microbiology 146, 23852394.[PubMed]
    [Google Scholar]
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