1887

Abstract

A novel Gram-negative, aerobic, psychrotolerant, alkali-tolerant, heterotrophic and dimorphic prosthecate bacterium, designated strain TAR-001, was isolated from deep-sea floor sediment in Japan. Cells of this strain had a dimorphic life cycle and developed an adhesive stalk at a site not coincident with the centre of the cell pole, and the other type of cell, a swarm cell, had a polar flagellum. Colonies were glossy, viscous and yellowish-white in colour. The temperature, pH and salt concentration range for growth were 2–41 °C, pH 6.5–10.0 and 1–4 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain TAR-001 belongs to the family of the class , and lies between the genus and the genus . Levels of similarity between the 16S rRNA gene sequence of strain TAR-001 and those of the type strains of species were 93.3–95.7 %; highest sequence similarity was with the type strain of . Levels of sequence similarity between those of the type strains of species were 94.9–96.0 %; highest sequence similarity was with the type strain of . The G+C content of strain TAR-001 was 67.6 mol%. Q-10 was the major respiratory isoprenoid quinone. The major fatty acids were C18 : 1ω7 and C16 : 0, and the presence of 1,2-di--acyl-3--[-glucopyranosyl-(1→4)-α--glucopyranuronosyl]glycerol suggests strain TAR-001 is more closely to the genus than to the genus . The mean DNA–DNA hybridization levels between strain TAR-001 and the type strains of two species of the genus were higher than that of the genus . On the basis of polyphasic biological features and the 16S rRNA gene sequence comparison presented here, strain TAR-001 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is TAR-001 ( = JCM 18150 = CECT 8073).

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2013-06-01
2024-03-28
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