1887

Abstract

A Gram-negative, motile and ovoid- to rod-shaped bacterial strain, designated M-M10, was isolated from a seashore sediment collected from the South Sea, South Korea. Strain M-M10 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M10 clustered with the type strains of , and , with which it exhibited sequence similarities of 97.4, 97.3 and 95.1 %, respectively. It exhibited 93.2–95.1 % sequence similarity to the type strains of the other species of the genus . Strain M-M10 contained Q-10 as the predominant ubiquinone and Cω7 and C as the major fatty acids, as observed in the genus . The polar lipid profile of strain M-M10 was similar to that of DSM 11457. The DNA G+C content of strain M-M10 was 63.0 mol% and its mean DNA–DNA relatedness values with DSM 16950 and MA1-10 were 16 % and 22 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, demonstrated that strain M-M10 is distinct from other species of the genus . On the basis of the data presented, strain M-M10 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-M10 ( = KCTC 23959 = CCUG 62413).

Funding
This study was supported by the:
  • Ministry of Education, Science and Technology (MEST) of the Republic of Korea
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2013-05-01
2024-03-29
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References

  1. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  2. Biebl H., Allgaier M., Lünsdorf H., Pukall R., Tindall B. J., Wagner-Döbler I. ( 2005 ). Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a . . Int J Syst Evol Microbiol 55, 23772383. [View Article] [PubMed]
    [Google Scholar]
  3. Boettcher K. J., Geaghan K. K., Maloy A. P., Barber B. J. ( 2005 ). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. . Int J Syst Evol Microbiol 55, 15311537. [View Article] [PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 2568. [View Article]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  7. Euzéby J. P. ( 1997 ). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590592. (List of Prokaryotic names with Standing in Nomenclature. Last full update: August 04, 2012. URL: http://www.bacterio.net). [CrossRef]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  9. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A. & other authors ( 2003 ). Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. . Int J Syst Evol Microbiol 53, 12611269. [View Article] [PubMed]
    [Google Scholar]
  10. Jung Y.-T., Lee J. S., Oh K.-H., Oh T.-K., Yoon J.-H. ( 2011 ). Roseovarius marinus sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 427432. [View Article] [PubMed]
    [Google Scholar]
  11. Kim Y.-O., Kong H. J., Park S., Kang S.-J., Kim W.-J., Kim K.-K., Oh T.-K., Yoon J.-H. ( 2012 ). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi . . Int J Syst Evol Microbiol 62, 931936. [View Article] [PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  13. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. ( 1999 ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. . Int J Syst Bacteriol 49, 137147. [View Article] [PubMed]
    [Google Scholar]
  14. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  15. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  18. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  19. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Wang B., Tan T., Shao Z. ( 2009 ). Roseovarius pacificus sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 59, 11161121. [View Article] [PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  23. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003 ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53, 449454. [View Article] [PubMed]
    [Google Scholar]
  25. Yoon J.-H., Kang S. J., Oh T. K. ( 2008 ). Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 58, 11981202. [View Article] [PubMed]
    [Google Scholar]
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