1887

Abstract

A species diversity study of lactic acid bacteria occurring in traditional Vietnamese nem chua yielded an isolate, LMG 26767, that could not be assigned to a species with a validly published name. The isolate was initially investigated by 16S rRNA gene sequence analysis, which revealed that it belonged to the genus , with and as the closest relatives (98.9 % and 96.9 % gene sequence similarity to the type strains, respectively). Comparative (GTG)-PCR genomic fingerprinting confirmed the unique taxonomic status of the novel strain. DNA–DNA hybridization experiments, DNA G+C content determination, sequence analysis of the phenylalanyl-tRNA synthase () gene, and physiological and biochemical characterization demonstrated that strain LMG 26767 represents a novel species, for which the name sp. nov. is proposed; the type strain is LMG 26767 ( = CCUG 62266). Biochemically, can be distinguished from and by its carbohydrate fermentation profile, absence of growth at 45 °C, and production of - and -lactate as end products of glucose metabolism.

Funding
This study was supported by the:
  • Belgian Technical Cooperation (BTC)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044123-0
2013-05-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/5/1754.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044123-0&mimeType=html&fmt=ahah

References

  1. De Bruyne K., Franz C. M. A. P., Vancanneyt M., Schillinger U., Mozzi F., de Valdez G. F., De Vuyst L., Vandamme P. ( 2008 ). Pediococcus argentinicus sp. nov. from Argentinean fermented wheat flour and identification of Pediococcus species by pheS, rpoA and atpA sequence analysis. . Int J Syst Evol Microbiol 58, 29092916. [View Article] [PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  3. Gevers D., Huys G., Swings J. ( 2001 ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. . FEMS Microbiol Lett 205, 3136. [View Article] [PubMed]
    [Google Scholar]
  4. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  5. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  6. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  7. Morlon-Guyot J., Guyot J. P., Pot B., De Haut I. J., Raimbault M. ( 1998 ). Lactobacillus manihotivorans sp. nov., a new starch-hydrolysing lactic acid bacterium isolated during cassava sour starch fermentation. . Int J Syst Bacteriol 48, 11011109. [View Article] [PubMed]
    [Google Scholar]
  8. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. ( 2005a ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151, 21412150. [View Article] [PubMed]
    [Google Scholar]
  9. Naser S. M., Vancanneyt M., De Graef E., Devriese L. A., Snauwaert C., Lefebvre K., Hoste B., Švec P., Decostere A. & other authors ( 2005b ). Enterococcus canintestini sp. nov., from faecal samples of healthy dogs. . Int J Syst Evol Microbiol 55, 21772182. [View Article] [PubMed]
    [Google Scholar]
  10. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. ( 2007 ). Identification of lactobacilli by pheS and rpoA gene sequence analyses. . Int J Syst Evol Microbiol 57, 27772789. [View Article] [PubMed]
    [Google Scholar]
  11. Nguyen H. T., Elegado F. B., Librojo-Basilio N. T., Mabesa R. C., Dizon E. I. ( 2010 ). Isolation and characterisation of selected lactic acid bacteria for improved processing of Nem chua, a traditional fermented meat from Vietnam. . Benef Microbes 1, 6774. [View Article] [PubMed]
    [Google Scholar]
  12. Nguyen T. L. D., Van Hoorde K., Cnockaert M., De Brandt E., De Bruyne K., Le T. B., Vandamme P. ( 2013 ). A culture-dependent and -independent approach for the identification of lactic acid bacteria associated with the production of nem chua, a Vietnamese fermented meat product. . Food Res Int 50, 232240. [CrossRef]
    [Google Scholar]
  13. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  14. Rantsiou K., Cocolin L. ( 2008 ). Fermented meat products. . In Molecular Techniques in the Microbial Ecology of Fermented Foods, pp. 91118. Edited by Cocolin L., Ercolini D. . New York:: Springer;. [View Article]
    [Google Scholar]
  15. Schumann P. ( 2011 ). Peptidoglycan structure. . In Methods in Microbiology Taxonomy of Prokaryotes, vol. 38, pp. 101129. Edited by Rainey F., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  16. Švec P., Vancanneyt M., Seman M., Snauwaert C., Lefebvre K., Sedlácek I., Swings J. ( 2005a ). Evaluation of (GTG)5-PCR for identification of Enterococcus spp. . FEMS Microbiol Lett 247, 5963. [View Article] [PubMed]
    [Google Scholar]
  17. Švec P., Vancanneyt M., Devriese L. A., Naser S. M., Snauwaert C., Lefebvre K., Hoste B., Swings J. ( 2005b ). Enterococcus aquimarinus sp. nov., isolated from sea water. . Int J Syst Evol Microbiol 55, 21832187. [View Article] [PubMed]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  19. Tanasupawat S., Pakdeeto A., Thawai C., Yukphan P., Okada S. ( 2007 ). Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov.. J Gen Appl Microbiol 53, 715. [View Article] [PubMed]
    [Google Scholar]
  20. Vancanneyt M., Naser S. M., Engelbeen K., De Wachter M., Van der Meulen R., Cleenwerck I., Hoste B., De Vuyst L., Swings J. ( 2006 ). Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov.. Int J Syst Evol Microbiol 56, 15531557. [View Article] [PubMed]
    [Google Scholar]
  21. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. ( 1994 ). Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). . Methods Mol Cell Biol 5, 2540.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044123-0
Loading
/content/journal/ijsem/10.1099/ijs.0.044123-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error