1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_8

gen. nov., sp. nov., a filamentous actinomycete of the family Free

Abstract

A novel filamentous actinomycete strain, designated TRM 46004, was isolated from sediment of Aiding Lake in Tulufan Basin (42° 64′ N 89° 26′ E), north-west China. The isolate was characterized using a polyphasic approach. The isolate formed abundant aerial mycelium with few branches and vegetative mycelium, occasionally twisted and coiled; spherical sporangia containing one to several spherical spores developed at the ends of short sporangiophores on aerial mycelium. The G+C content of the DNA was 65.2 mol%. The isolate contained -diaminopimelic acid as the diagnostic diamino acid and xylose, galactose and ribose as the major whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C and anteiso-C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 46004 formed a distinct lineage within the family and showed 91.7–96.1 % 16S rRNA gene sequence similarity with members of the family . On the basis of the evidence from this polyphasic study, a novel genus and species, gen. nov., sp. nov., are proposed. The type strain of is TRM 46004 ( = CGMCC 4.5737 = NBRC 107726).

  • Published Online:
Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB134505)
  • National High Technology Research and Development Program of China (Award 2012AA021705)
  • Chinese Academy of Sciences (Award SKLMR-20090603)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.044222-0
2013-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/8/2813.html?itemId=/content/journal/ijsem/10.1099/ijs.0.044222-0&mimeType=html&fmt=ahah

References

  1. Chun J., Goodfellow M. ( 1995 ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45, 240–245. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  3. Embley M. T., Smida J., Stackebrandt E. ( 1988 ). The phylogeny of mycolate-less wall chemotype IV actinomycetes and description of Pseudonocardiaceae fam. nov.. Syst Appl Microbiol 11, 44–52. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  7. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 1129–1133. [View Article] [PubMed]
    [Google Scholar]
  8. Hasegawa T. ( 1988 ). Actinokineospora: a new genus of the Actinomycetales . . Actinomycetologica 2, 31–45. [View Article]
    [Google Scholar]
  9. Hasegawa T., Lechevalier M. P., Lechevalier H. A. ( 1978 ). A new genus of Actinomycetales: Actinosynnema gen. nov.. Int J Syst Bacteriol 28, 304–310. [View Article]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  11. Henssen A. ( 1957 ). Beiträge zur Morphologie und Systematik der thermophilen Actinomyceten. . Arch Mikrobiol 26, 373–414. (in German). [View Article] [PubMed]
    [Google Scholar]
  12. Huang Y., Wang L., Lu Z., Hong L., Liu Z., Tan G. Y. A., Goodfellow M. ( 2002 ). Proposal to combine the genera Actinobispora and Pseudonocardia in an emended genus Pseudonocardia, and description of Pseudonocardia zijingensis sp. nov.. Int J Syst Evol Microbiol 52, 977–982. [View Article] [PubMed]
    [Google Scholar]
  13. Indananda C., Matsumoto A., Inahashi Y., Takahashi Y., Duangmal K., Thamchaipenet A. ( 2010 ). Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 60, 1141–1146. [View Article] [PubMed]
    [Google Scholar]
  14. Jiang Y., Wiese J., Tang S. K., Xu L. H., Imhoff J. F., Jiang C. L. ( 2008 ). Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 58, 408–413. [View Article] [PubMed]
    [Google Scholar]
  15. Kelly K. L. ( 1964 ). Inter-Society Color Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  16. Kim S. B., Goodfellow M. ( 1999 ). Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov.. Int J Syst Bacteriol 49, 507–512. [View Article] [PubMed]
    [Google Scholar]
  17. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  18. Korn-Wendisch F., Rainey F., Kroppenstedt R. M., Kempf A., Majazza A., Kutzner H. J., Stackebrandt E. ( 1995 ). Thermocrispum gen. nov., a new genus of the order Actinomycetales, and description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.. Int J Syst Bacteriol 45, 67–77. [View Article] [PubMed]
    [Google Scholar]
  19. Labeda D. P. ( 2001 ). Crossiella gen. nov., a new genus related to Streptoalloteichus . . Int J Syst Evol Microbiol 51, 1575–1579.[PubMed] [CrossRef]
    [Google Scholar]
  20. Labeda D. P., Kroppenstedt R. M. ( 2006 ). Goodfellowia gen. nov., a new genus of the Pseudonocardineae related to Actinoalloteichus, containing Goodfellowia coeruleoviolacea gen. nov., comb. nov.. Int J Syst Evol Microbiol 56, 1203–1207. [View Article] [PubMed]
    [Google Scholar]
  21. Labeda D. P., Kroppenstedt R. M. ( 2007 ). Proposal of Umezawaea gen. nov., a new genus of the Actinosynnemataceae related to Saccharothrix, and transfer of Saccharothrix tangerinus Kinoshita et al. 2000 as Umezawaea tangerina gen. nov., comb. nov.. Int J Syst Evol Microbiol 57, 2758–2761. [View Article] [PubMed]
    [Google Scholar]
  22. Labeda D. P., Kroppenstedt R. M. ( 2008 ). Proposal for the new genus Allokutzneria gen. nov. within the suborder Pseudonocardineae and transfer of Kibdelosporangium albatum Tomita et al. 1993 as Allokutzneria albata comb. nov.. Int J Syst Evol Microbiol 58, 1472–1475. [View Article] [PubMed]
    [Google Scholar]
  23. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. ( 1984 ). Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis . . Int J Syst Bacteriol 34, 426–431. [View Article]
    [Google Scholar]
  24. Labeda D. P., Hatano K., Kroppenstedt R. M., Tamura T. ( 2001 ). Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.. Int J Syst Evol Microbiol 51, 1045–1050. [View Article] [PubMed]
    [Google Scholar]
  25. Labeda D. P., Goodfellow M., Chun J., Zhi X. Y., Li W. J. ( 2011 ). Reassessment of the systematics of the suborder Pseudonocardineae: transfer of the genera within the family Actinosynnemataceae Labeda and Kroppenstedt 2000 emend. Zhi et al. 2009 into an emended family Pseudonocardiaceae Embley et al. 1989 emend. Zhi et al. 2009. . Int J Syst Evol Microbiol 61, 1259–1264. [View Article] [PubMed]
    [Google Scholar]
  26. Lacy J., Goodfellow M. ( 1975 ). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov.. J Gen Microbiol 88, 75–85. [View Article] [PubMed]
    [Google Scholar]
  27. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J. S. ( 1986 ). Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36, 29–37. [View Article]
    [Google Scholar]
  28. Mao J., Wang J., Dai H. Q., Zhang Z. D., Tang Q. Y., Ren B., Yang N., Goodfellow M., Zhang L. X., Liu Z. H. ( 2011 ). Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae . . Int J Syst Evol Microbiol 61, 621–630. [View Article] [PubMed]
    [Google Scholar]
  29. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  30. Nonomura H., Ohara Y. ( 1971 ). Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes. . J Ferment Technol 49, 895–903.
     [Google Scholar]
  31. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  32. Shearer M. C., Colman P. M., Ferrin R. M., Nisbet L. J., Nash C. H. ( 1986 ). New genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov.. Int J Syst Bacteriol 36, 47–54. [View Article]
    [Google Scholar]
  33. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  34. Stackebrandt E., Kroppenstedt R. M., Jahnke K. D., Kemmerling C., Gurtler H. ( 1994 ). Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. Kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb. nov., Kutzneria kofuensis comb. nov., and Kutzneria albida comb. nov., respectively, and emendation of the genus Streptosporangium . . Int J Syst Bacteriol 44, 265–269. [View Article]
    [Google Scholar]
  35. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997 ). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47, 479–491. [View Article]
    [Google Scholar]
  36. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  37. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  38. Tamura T., Zhiheng L., Yamei Z., Hatano K. ( 2000 ). Actinoalloteichus cyanogriseus gen. nov., sp. nov.. Int J Syst Evol Microbiol 50, 1435–1440. [View Article] [PubMed]
    [Google Scholar]
  39. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  40. Tang S. K., Wang Y., Zhang H., Lee J. C., Lou K., Kim C. J., Li W. J. ( 2010 ). Haloechinothrix alba gen. nov., sp. nov., a halophilic, filamentous actinomycete of the suborder Pseudonocardineae . . Int J Syst Evol Microbiol 60, 2154–2158. [View Article] [PubMed]
    [Google Scholar]
  41. Tian X. P., Zhi X. Y., Qiu Y. Q., Zhang Y. Q., Tang S. K., Xu L. H., Zhang S., Li W. J. ( 2009 ). Sciscionella marina gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. . Int J Syst Evol Microbiol 59, 222–228. [View Article] [PubMed]
    [Google Scholar]
  42. Tomita K., Nakakita Y., Hoshino Y., Numata K., Kawaguchi H. ( 1987 ). New genus of the Actinomycetales: Streptoalloteichus hindustanus gen. nov., nom. rev.; sp. nov., nom. rev.. Int J Syst Bacteriol 37, 211–213. [View Article]
    [Google Scholar]
  43. Wang Y., Zhang Z., Ruan J. S. ( 1996 ). A proposal to transfer Microbispora bispora (Lechevalier 1965) to a new genus, Thermobispora gen. nov., as Thermobispora bispora comb. nov.. Int J Syst Bacteriol 46, 933–938. [View Article] [PubMed]
    [Google Scholar]
  44. Williams S. T., Goodfellow M., Alderson G. ( 1989 ). Genus Streptomyces Waksman and Henrici 1943, 339AL . . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  45. Xia Z. F., Guan T. W., Ruan J. S., Huang Y., Zhang L. L. ( 2011 ). [Studies on the actinobacterial diversity in aiding lake sediments]. . Wei Sheng Wu Xue Bao 51, 1023–1031 (in Chinese).[PubMed]
    [Google Scholar]
  46. Yassin A. F., Rainey F. A., Brzezinka H., Jahnke K. D., Weissbrodt H., Budzikiewicz H., Stackebrandt E., Schaal K. P. ( 1995 ). Lentzea gen. nov., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 45, 357–363. [View Article] [PubMed]
    [Google Scholar]
  47. Yuan L. J., Zhang Y. Q., Yu L. Y., Liu H. Y., Guan Y., Lee J. C., Kim C. J., Zhang Y. Q. ( 2010 ). Alloactinosynnema album gen. nov., sp. nov., a member of the family Actinosynnemataceae isolated from soil. . Int J Syst Evol Microbiol 60, 39–43. [View Article] [PubMed]
    [Google Scholar]
  48. Zhi X. Y., Li W. J., Stackebrandt E. ( 2009 ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59, 589–608. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.044222-0
Loading
Longimycelium tulufanense gen. nov., sp. nov., a filamentous actinomycete of the family Pseudonocardiaceae
Int J Syst Evol Microbiol 63, 2813 (2013); https://doi.org/10.1099/ijs.0.044222-0
/content/journal/ijsem/10.1099/ijs.0.044222-0
/content/journal/ijsem/10.1099/ijs.0.044222-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error