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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_5

sp. nov., isolated from mangrove sediment Free

Abstract

A Gram-negative, short-rod-shaped and non-motile bacterium, strain 12C11, was isolated from an oil-degrading consortium, enriched from the Fugong mangrove sediment, Fujian Province of China. Optimum growth was observed at 25 °C, at pH 7.5 and with 4 % (w/v) NaCl. Comparative 16S rRNA gene sequence analysis demonstrated that it shared highest similarity with members of the genus (97.5–93.4 %), exhibiting 97.5 % sequence similarity to IMSNU 14014, followed by KMM 6241 (97.2 %); it shared <96.0 % sequence similarity with other members of the genus. Levels of nucleotide sequence similarity between (DNA gyrase subunit B) genes of strain 12C11 and KMM 6241, IMSNU 14014, SW-2 and 7-UAH were 78.8, 78.6, 75.6 and 77.4 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain 12C11 and other strains formed a distinct lineage. The dominant fatty acids were iso-C (32.1 %), Cω7/Cω6 (11.6 %), iso-C I/anteiso-C B (10.1 %), iso-C 3-OH (9.2 %) and iso-Cω9/C 10-methyl (7.1 %), which accounted for 70.0 % of the total fatty acids. DNA–DNA hybridization showed that strain 12C11 shared low DNA–DNA relatedness with KMM 6241 and IMSNU 14014 (30.7±0.9 and 30.5±1.8 %, respectively). The G+C content of the chromosomal DNA of strain 12C11 was 38.4 mol%. The major respiratory quinones were MK-7 (96.0 %) and MK-6 (4.0 %). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, the novel strain most appropriately belongs to the genus , but can readily be distinguished from known species. The name sp. nov. is proposed (type strain 12C11 = CGMCC 1.11027 = MCCC 1F01099 = DSM 25035).

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Funding
This study was supported by the:
  • National Science Foundation of China (Award 30930017, 41176093 and 40976069)
© 2013 IUMS
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2013-05-01
2024-04-24
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Algoriphagus zhangzhouensis sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 63, 1621 (2013); https://doi.org/10.1099/ijs.0.044271-0
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