RT Journal Article SR Electronic(1) A1 Lang, Elke A1 Schumann, Peter A1 Adler, Susann A1 Spröer, Cathrin A1 Sahin, NurettinYR 2013 T1 Azorhizobium oxalatiphilum sp. nov., and emended description of the genus Azorhizobium JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_4 SP 1505 OP 1511 DO https://doi.org/10.1099/ijs.0.045229-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative, motile, non-spore-forming rod, designated NS12T, was isolated from macerated petioles of Rumex sp. after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS12T was phylogenetically related to the genera Azorhizobium and Xanthobacter in the class Alphaproteobacteria . Strain NS12T was most closely related to Azorhizobium doebereinerae BR 5401T and Azorhizobium caulinodans ORS 571T (98.3 and 97.3 % 16S rRNA gene sequence similarity, respectively). Membership of the genus Xanthobacter was excluded by phenotypic characterization. The whole-cell fatty acid compositions of the isolate was typical of members of the genus Azorhizobium with C18 : 1ω7c, cyclo-C19 : 0ω8c, 11-methyl-C18 : 1ω7c and C16 : 0 as the main components. The results of DNA–DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strain NS12T from the two members of the genus Azorhizobium . Therefore it is concluded that the isolate represents a novel species of the genus Azorhizobium , for which the name Azorhizobium oxalatiphilum sp. nov. is proposed. The type strain is NS12T ( = DSM 18749T = CCM 7897T). The description of the genus Azorhizobium is also emended., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.045229-0