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Volume 63, Issue Pt_6

sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil Free

Abstract

A novel biosurfactant-producing strain, designated YW1, was isolated from agricultural soil. Its taxonomic position was investigated using a polyphasic approach. The cells were short rods, Gram-negative, non-sporulating and motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YW1 was a member of the genus , and showed highest sequence similarities to LMG 2370 (98.5 %), LMG 3475 (97.7 %) and LMG 1253 (97.7 %). Furthermore, DNA–DNA hybridization experiments against these three strains gave results that were clearly lower than 70 % DNA–DNA similarity, and consequently confirmed that this new strain does not belong to a previously described species of the genus . The major respiratory quinone was ubiquinone-8. The major fatty acids (>5 %) were C (30.1 %), summed feature 3 (Cω6 and/or Cω7; 25.4 %), summed feature 8 (Cω6 and/or Cω7; 15.3 %), C cyclo (7.4 %) and C (5.8 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown phospholipids and unknown lipids. Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain YW1 was clearly distinguishable from all species of the genus with validly published names and should be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YW1 ( = CCTCC AB 2012033 = KACC 16697).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200087)
  • Natural Science Foundation of Jiangsu Province (Award BK2011066)
  • Fundamental Research Funds for the Central Universities (Award KYZ201122)
  • Grand Science and Technology Special Project of the Chinese Academy of Tropical Agricultural Sciences (Award 2010hzsZDZX001)
© 2013 IUMS
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2013-06-01
2024-04-18
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Comamonas jiangduensis sp. nov., a biosurfactant-producing bacterium isolated from agricultural soil
Int J Syst Evol Microbiol 63, 2168 (2013); https://doi.org/10.1099/ijs.0.045716-0
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