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Volume 63, Issue Pt_6

sp. nov., a halophilic archaeon isolated from a lake Free

Abstract

Two extremely halophilic archaea, designated YIM 94188 and YIM 94189, were isolated from Qijiaojing lake in Xinjiang province, north-west China and subjected to taxonomic characterization using a polyphasic approach. The cells of the two strains were coccoid, non-motile and Gram-stain-negative. Colonies were pink–white-pigmented and aerobic. Growth occurred at 10–30 % (w/v) NaCl, 20–55 °C and pH 6.0–8.0 (optimum: 20–25 % NaCl, 37–42 °C, pH 6.5–7.0). Magnesium was necessary for growth in the range of 0.2–1.2 M. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains belonged to the genus showing 98.5 % sequence similarity to the closest phylogenetic neighbour, RO5-2. In addition, the DNA–DNA hybridization values of strains YIM 94188 and YIM 94189 to RO5-2 were 35.7 % and 37.7 %, respectively. Polar lipid analyses revealed that the two strains contained phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The DNA G+C contents of strains YIM 94188 and YIM 94189 were 66.3 mol% and 64.6 mol%, respectively. On the basis of physiological and chemotaxonomic data, and phylogenetic analysis, strains YIM 94188 and YIM 94189 were classified as representing a novel species in the genus . The name sp. nov. is proposed, with YIM 94188 ( = CCTCC AB 2010456 = JCM 17506) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National High-tech R&D Program of China (863 Program) (Award 2012AA021705)
  • National Natural Science Foundation of China (Award 201200008)
  • West Light Foundation of the Chinese Academy of Sciences
  • Chinese Academy of Sciences
© 2013 IUMS
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2013-06-01
2024-04-24
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Halopelagius fulvigenes sp. nov., a halophilic archaeon isolated from a lake
Int J Syst Evol Microbiol 63, 2192 (2013); https://doi.org/10.1099/ijs.0.045773-0
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