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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_6

sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar Free

Abstract

Two helical-shaped bacteria (strains JC133 and JC143), which stain Gram-negative, were isolated from an alkaline soda lake, Lonar, India. Both strains were obligate anaerobes, mesophilic and required halo-alkaline conditions for growth. Both strains were resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Both strains had phosphatidylglycerol (PG), diphosphotidylglycerol (DPG), glycolipid (GL) and four unidentified lipids (L1–4) as the major polar lipids. Cω7 was the predominant cellular fatty acid with significant proportions of C, Cω9, C, C, Cω5, Cω5 and Cω9. The DNA G+C contents of strain JC131 and JC143 were 58.2 and 58.5 mol%, respectively, and the two strains showed DNA reassociation >85 % (based on DNA–DNA hybridization). Based on the 16S rRNA gene sequence analysis, both strains were identified as belonging to the genus with Z-7491 (99.6 % sequence similarity), ASpG1 (99 %) and other members of the genus (<93 %) as their closest phylogenetic neighbours. However, strain JC133 and JC143 displayed less than 53.5 % binding (based on DNA–DNA hybridization) with Z-7491 and ASpG1. On the basis of physiological, biochemical, chemotaxonomic and molecular properties, strains JC133 and JC143 can be differentiated from other members of the genus and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC133 ( = KCTC 15220 = NBRC 109056).

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Funding
This study was supported by the:
  • Department of Biotechnology, Government of India
  • University Grants Commission (UGC)
© 2013 IUMS
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2013-06-01
2024-04-19
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Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar
Int J Syst Evol Microbiol 63, 2223 (2013); https://doi.org/10.1099/ijs.0.046292-0
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