@article{mbs:/content/journal/ijsem/10.1099/ijs.0.046433-0, author = "Choi, Ahyoung and Yang, Seung-Jo and Rhee, Kwang-Hyun and Cho, Jang-Cheon", title = "Lentisphaera marina sp. nov., and emended description of the genus Lentisphaera", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_4", pages = "1540-1544", doi = "https://doi.org/10.1099/ijs.0.046433-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.046433-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two Gram-stain-negative, non-motile, non-pigmented cocci, designated IMCC11369T and IMCC11389, were isolated from surface seawater of the East Sea of Korea by high-throughput cultivation based on dilution to extinction. Strains IMCC11369T and IMCC11389 shared 99.9 % 16S rRNA gene sequence similarity and >86.3 % DNA–DNA relatedness, which suggested that they belong to the same genomic species. The isolates were most closely related to Lentisphaera araneosa HTCC2155T (99.0 % 16S rRNA gene sequence similarity). Phylogenetic analysis revealed that the isolates formed a robust cluster with L. araneosa HTCC2155T. DNA–DNA relatedness values, however, showed that the isolates were distantly related to L. araneosa HTCC2155T (2.0–18.6 %), which suggested that they represent a separate genomic species in the genus Lentisphaera . The two isolates were phenotypically differentiated from their closest relative by several characteristics, including degradation of macromolecules and carbon source utilization. The DNA G+C content was 44.5–45.2 mol% and the predominant cellular fatty acids were C14 : 0, C16 : 1ω9c and C16 : 0. Strain IMCC11369T contained MK-7 as the respiratory quinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids. On the basis of data obtained in this study, a novel species is proposed to accommodate the isolates, Lentisphaera marina sp. nov. The type strain is IMCC11369T ( = KCTC 23780T = NBRC 108776T).", }