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Volume 63, Issue Pt_8

sp. nov., an actinomycete from soil Free

Abstract

A novel actinomycete, strain FX61, was isolated from a saline sample collected from the Inner Mongolian Autonomous Region in China and subjected to a taxonomic study using a polyphasic approach. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C, anteiso-C, iso-C, iso-C H, C, iso-C and anteiso-C. The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides and an unidentified phospholipid. The G+C content of the genomic DNA was 72 mol%. The 16S rRNA gene sequence of the isolate had greater than 98 % similarity with those of ATCC 23623 (98.2 %), DSM 41446 (98.2 %), ATCC 19815 (98.2 %), ATCC 27427 (98.2 %), ATCC 23935 (98.2 %), ATCC 19724 (98.2 %) and ATCC 33049 (98.0 %), showing that the novel strain should be assigned to the genus . DNA–DNA hybridizations with the seven above-mentioned members of the genus showed 29.8, 28.5, 27.0, 25.5, 25.0, 23.5 and 22.0 % relatedness, respectively. On the basis of the phenotypic characteristics and genotypic distinctiveness, strain FX61 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FX61 ( = CGMCC 4.7042 = KCTC 29112).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31070002 and 30970009)
  • Special Fund for Public Welfare Industrial (Agriculture) Research of China (Award 200903001)
© 2013 IUMS
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2013-08-01
2024-04-16
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 3389–3402. [View Article] [PubMed]
    [Google Scholar]
  2. Anderson A. S., Wellington E. M. H. ( 2001 ). The taxonomy of Streptomyces and related genera. . Int J Syst Evol Microbiol 51, 797–814. [View Article] [PubMed]
    [Google Scholar]
  3. Chun J. S., Youn H. D., Yim Y. I., Lee H. K., Kim M. Y., Hah Y. C., Kang S. O. ( 1997 ). Streptomyces seoulensis sp. nov.. Int J Syst Bacteriol 47, 492–498. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M. ( 1987 ). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis . . FEMS Microbiol Lett 41, 35–39. [View Article]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  6. Goodfellow M. ( 1971 ). Numerical taxonomy of some nocardioform bacteria. . J Gen Microbiol 69, 33–80. [View Article] [PubMed]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 54–63. [View Article]
    [Google Scholar]
  8. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  9. Huß V. A., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  10. Jahnke K.-D. ( 1992 ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15, 61–73. [View Article]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  12. Kelly K. L. ( 1964 ). Inter-Society Color Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  13. Kim B. S., Hwang B. K. ( 2003 ). Biofungicides. . In Fungal Biotechnology in Agricultural, Food and Environmental Applications, pp. 123–133. Edited by Arora D. K. . New York:: Marcel Dekker;.
     [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  15. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  16. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 1–32. [View Article]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435–443. [View Article]
    [Google Scholar]
  18. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 1424–1428. [View Article] [PubMed]
    [Google Scholar]
  19. Locci R. ( 1989 ). Streptomyces and related genera. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2451–2508. Edited by Williams S. T., Sharpe M. E., Holt J. G. . Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  20. Marmur J., Doty P. ( 1962 ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5, 109–118. [View Article] [PubMed]
    [Google Scholar]
  21. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 87–95. [View Article]
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acid, Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  24. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. ( 1968a ). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. . Int J Syst Bacteriol 18, 69–189. [View Article]
    [Google Scholar]
  26. Shirling E. B., Gottlieb D. ( 1968b ). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. . Int J Syst Bacteriol 18, 279–392. [View Article]
    [Google Scholar]
  27. Shirling E. B., Gottlieb D. ( 1969 ). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. . Int J Syst Bacteriol 19, 391–512. [View Article]
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  31. Waksman S. A., Henrici A. T. ( 1943 ). The nomenclature and classification of the actinomycetes. . J Bacteriol 46, 337–341.[PubMed]
    [Google Scholar]
  32. Wan L., Wang J. Q., Yang S. Y., Wang N. B. ( 2006 ). Antibacterial Activity of Intestinal Bacterial in Prawn (Litepenaeus vannamei). . Fisheries Science 25, 62–64.
     [Google Scholar]
  33. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813.[PubMed]
    [Google Scholar]
  34. Zhao X. Q., Li W. J., Jiao W. C., Li Y., Yuan W. J., Zhang Y. Q., Klenk H. P., Suh J. W., Bai F. W. ( 2009 ). Streptomyces xinghaiensis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 59, 2870–2874. [View Article] [PubMed]
    [Google Scholar]
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Streptomyceswuyuanensis sp. nov., an actinomycete from soil
Int J Syst Evol Microbiol 63, 2945 (2013); https://doi.org/10.1099/ijs.0.047050-0
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