%0 Journal Article %A Saito, Mitsumasa %A Villanueva, Sharon Y. A. M. %A Kawamura, Yoshiaki %A Iida, Ken-ichiro %A Tomida, Junko %A Kanemaru, Takaaki %A Kohno, Eri %A Miyahara, Satoshi %A Umeda, Akiko %A Amako, Kazunobu %A Gloriani, Nina G. %A Yoshida, Shin-ichi %T Leptospiraidonii sp. nov., isolated from environmental water %D 2013 %J International Journal of Systematic and Evolutionary Microbiology, %V 63 %N Pt_7 %P 2457-2462 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.047233-0 %I Microbiology Society, %X Strain Eri-1T was isolated from a water sample on the campus of Kyushu University, Fukuoka, Japan. The motility and morphology of the isolate were similar to those of members of the genus Leptospira , but the spiral structure of the isolate was sharper under dark-field microscopy. Cells were 10.6±1.3 µm long and 0.2 µm in diameter, with a wavelength of 0.9 µm and an amplitude of 0.4 µm. Strain Eri-1T grew in Korthof’s medium at both 13 and 30 °C, and also in the presence of 8-azaguanine. 16S rRNA gene-based phylogenetic analysis placed strain Eri-1T within the radiation of the genus Leptospira where it formed a unique lineage within the clade of the known saprophytic species of the genus Leptospira . The strain was not pathogenic to hamsters. Strain Eri-1T exhibited low levels (11.2–12.6 %) of similarity by DNA–DNA hybridization to the three most closely related species of the genus Leptospira . The DNA G+C content of the genome of strain Eri-1T was 42.5±0.1 mol%. These results suggest that strain Eri-1T represents a novel species of the genus Leptospira , for which the name Leptospira idonii sp. nov. is proposed. The type strain is Eri-1T ( = DSM 26084T = JCM 18486T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.047233-0