Microbacteriumsaccharophilum sp. nov., isolated from a sucrose-refining factory
Ohta, Yukari and Ito, Tetsuya and Mori, Kozue and Nishi, Shinro and Shimane, Yasuhiro and Mikuni, Katsuhiko and Hatada, Yuji,, 63, 2765-2769 (2013),
doi = https://doi.org/10.1099/ijs.0.047258-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-stain-positive, non-motile, non-spore-forming, rod-shaped bacterium, designated strain K-1T, was isolated from soil at a sucrose refinery in Japan. The strain grew at 9–37 °C (optimum, 30 °C) and at pH 6–11 (optimum, pH 7.0). Phylogenetic analysis based on the full-length 16S rRNA gene sequence of strain K-1T revealed that it was a member of the genus Microbacterium . High 16S rRNA gene sequence similarities were found between strains K-1T and both Microbacterium pumilum NBRC 101279T (99.7 %) and Microbacterium deminutum NRRL B-24453T (99.5 %). However, the DNA–DNA hybridization values between strain K-1T and M. pumilum NBRC 101279T and M. deminutum NRRL B-24453T were only 12 % and 10 %, respectively. The DNA G+C content of strain K-1T was 73 mol%. The major fatty acids of strain K-1T were anteiso-C15 : 0 and anteiso-C17 : 0, and the major menaquinones were MK-12 and MK-13. The diamino acid in the cell-wall peptidoglycan was lysine. On the basis of these results, strain K-1T is considered to represent a novel species of the genus Microbacterium , for which the name Microbacterium saccharophilum sp. nov. is proposed. The type strain is K-1T ( = NBRC 108778T = NCIMB 14782T).,
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