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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

sp. nov., isolated from a marine tidal flat Free

Abstract

A novel Gram-stain-negative, heterotrophic, moderate halophilic and strictly aerobic bacterium, strain 105, was isolated from a tidal flat of Taean in Korea. Cells were catalase- and oxidase-positive long rods that showed gliding motility. Optimum temperature, pH and salinity for the growth of strain 105 were observed at 30–37 °C, at pH 7.0–7.5, and in the presence of 2–4 % (w/v) NaCl, respectively. The major cellular fatty acids were iso-C G, iso-C and iso-C 3-OH. Phosphatidylethanolamine and five unidentified lipids were identified as the major polar lipids. The genomic DNA G+C content of strain 105 was 42.4 mol% and MK-6 was detected as the predominant isoprenoid quinone. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 105 formed a phyletic lineage with members of the genus . Strain 105 was most closely related to SW-63 (97.6 %), BB-My12 (97.5 %), SMK-108 (97.5 %), B1 (97.3 %), CL-SS4 (97.2 %) and (96.2 %). The DNA–DNA relatedness values of strain 105 with JCM 11811, KCTC 23501, KCTC 22173, DSM 13258 and JCM 11812 were 17.2±6.0, 8.7±2.2, 3.7±0.5, 11.0±1.9 and 7.1±1.3 %, respectively. On the basis of phenotypic and molecular features, strain 105 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 105 ( = KACC 16195 = JCM 17757).

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Funding
This study was supported by the:
  • National Research Foundation of Korea grant (Award 2012R1A1B)
  • Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR), Korea
© 2013 IUMS
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2013-07-01
2024-04-16
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References

  1. Arun A. B., Chen W.-M., Lai W.-A., Chao J.-H., Rekha P. D., Shen F.-T., Singh S., Young C.-C. ( 2009 ). Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. . Int J Syst Evol Microbiol 59, 2738–2742. [View Article] [PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  4. DeSantis T. Z. Jr, Hugenholtz P., Keller K., Brodie E. L., Larsen N., Piceno Y. M., Phan R., Andersen G. L. ( 2006 ). nast: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. . Nucleic Acids Res 34 (Web Server issue), W394–W399. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 2002 ). phylip (phylogeny inference package). , version 3.6a, Seattle: Department of Genetics, University of Washington;, Seattle, WA, USA:.
     [Google Scholar]
  6. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . In Methods in Enzymology, vol. 1, pp. 138–146. Edited by Colowick S. P., Kaplan N. O. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  7. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770–773. [View Article] [PubMed]
    [Google Scholar]
  8. Hwang C. Y., Kim M. H., Bae G. D., Zhang G. I., Kim Y. H., Cho B. C. ( 2009 ). Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. . Int J Syst Evol Microbiol 59, 1856–1861. [View Article] [PubMed]
    [Google Scholar]
  9. Jeon C. O., Park W., Ghiorse W. C., Madsen E. L. ( 2004 ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54, 93–97. [View Article] [PubMed]
    [Google Scholar]
  10. Jin H. M., Jeong H., Moon E.-J., Math R. K., Lee K., Kim H.-J., Jeon C. O., Oh T. K., Kim J. F. ( 2011a ). Complete genome sequence of the polycyclic aromatic hydrocarbon-degrading bacterium Alteromonas sp. strain SN2. . J Bacteriol 193, 4292–4293. [View Article] [PubMed]
    [Google Scholar]
  11. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O. ( 2011b ). Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 1392–1396. [View Article] [PubMed]
    [Google Scholar]
  12. Jin H. M., Kim J. M., Lee H. J., Madsen E. L., Jeon C. O. ( 2012 ). Alteromonas as a key agent of polycyclic aromatic hydrocarbon biodegradation in crude oil-contaminated coastal sediment. . Environ Sci Technol 46, 7731–7740. [View Article] [PubMed]
    [Google Scholar]
  13. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O. ( 2011 ). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 1534–1538. [View Article] [PubMed]
    [Google Scholar]
  14. Kim J. M., Lee S. H., Jung J. Y., Jeon C. O. ( 2010 ). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 60, 1828–1831. [View Article] [PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  17. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 1–67. [View Article]
    [Google Scholar]
  18. Lee S. H., Shim J. K., Kim J. M., Choi H. K., Jeon C. O. ( 2011 ). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . . Int J Syst Evol Microbiol 61, 722–727. [View Article] [PubMed]
    [Google Scholar]
  19. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. ( 2012 ). Muricauda beolgyonensis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 62, 1134–1139. [View Article] [PubMed]
    [Google Scholar]
  20. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184. [PubMed]
    [Google Scholar]
  21. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. ( 2006 ). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44, 155–161. [PubMed]
    [Google Scholar]
  22. Math R. K., Jin H. M., Kim J. M., Hahn Y., Park W., Madsen E. L., Jeon C. O. ( 2012 ). Comparative genomics reveals adaptation by Alteromonas sp. SN2 to marine tidal-flat conditions: cold tolerance and aromatic hydrocarbon metabolism. . PLoS ONE 7, e35784. [View Article] [PubMed]
    [Google Scholar]
  23. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  24. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O. ( 2011 ). Luteimonas lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61, 2729–2733. [View Article] [PubMed]
    [Google Scholar]
  25. Rosselló-Mora R., Amann R. ( 2001 ). The species concept for prokaryotes. . FEMS Microbiol Rev 25, 39–67. [View Article] [PubMed]
    [Google Scholar]
  26. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  27. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. . Washington, D.C.:: American Society for Microbiology;.
     [Google Scholar]
  28. Stamatakis, A., Ott, M. & Ludwig, T. (2005). RAxML-OMP: An efficient program for phylogenetic inference on SMPs”. In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science, 3506, 288–302, Springer Verlag.
  29. Stevens H., Brinkhoff T., Rink B., Vollmers J., Simon M. ( 2007 ). Diversity and abundance of Gram positive bacteria in a tidal flat ecosystem. . Environ Microbiol 9, 1810–1822. [View Article] [PubMed]
    [Google Scholar]
  30. Wang Q., Garrity G. M., Tiedje J. M., Cole J. R. ( 2007 ). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol 73, 5261–5267. [View Article] [PubMed]
    [Google Scholar]
  31. Yoon J.-H., Lee M.-H., Oh T.-K., Park Y.-H. ( 2005 ). Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda . . Int J Syst Evol Microbiol 55, 1015–1019. [View Article] [PubMed]
    [Google Scholar]
  32. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2008 ). Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 58, 1603–1607. [View Article] [PubMed]
    [Google Scholar]
  33. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad’on N., Gorlenko V. M., Kompantseva E. I., Drews G. ( 1994 ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. . Int J Syst Bacteriol 44, 427–434. [View Article] [PubMed]
    [Google Scholar]
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Muricaudataeanensis sp. nov., isolated from a marine tidal flat
Int J Syst Evol Microbiol 63, 2672 (2013); https://doi.org/10.1099/ijs.0.047647-0
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