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Volume 63, Issue Pt_8

gen. nov., sp. nov., a member of the family isolated from the Bashi Channel Free

Abstract

A Gram-reaction-negative, non-spore-forming, gliding, non-translucent, colourless or yellow, aerobic and elevated-colony-forming strain, designated E403, was isolated from the Bashi Channel and subjected to a polyphasic taxonomic study. Strain E403 could grow in the presence of 0.3–8 % (w/v) NaCl, at 16–43 °C and at pH 6–9, and grew optimally at 28 °C, pH 8, in natural seawater medium. The respiratory quinones were MK-6 and MK-7. The major fatty acids were iso-C, iso-C 3-OH, iso-C G, summed feature 3 (Cω7/Cω6), iso-C 3-OH and C. The DNA G+C content of strain E403 was 37.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences of members of the family showed that strain E403 formed a distinct evolutionary lineage within the stable cluster containing type strains HN-171 (92.2 % similarity) and En5 (92.4 % similarity). In addition to the large 16S rRNA gene sequence differences, E403 can also be distinguished from the reference type strains En5 and SCSIO 03483 by several phenotypic characteristics and chemotaxonomic properties. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain E403 is suggested to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is E403 ( = CCTCC AB2012862 = CGMCC 1.12333 = KCTC 32192).

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award 41206136)
  • Guangdong Province and Chinese Academy of Science cooperation Foundation (Award 2012B091100276)
  • Public Science and Technology Research Funds Projects of Ocean (Award 201005031-5)
  • Provincial Collaborative Foundation Project of Guangdong (Award 9351007002000001)
  • Key Laboratory of Marine Bio-resources Sustainable Utilization, Chinese Academy of Science
© 2013 IUMS
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2013-08-01
2024-04-19
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Pustulibacterium marinum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the Bashi Channel
Int J Syst Evol Microbiol 63, 3056 (2013); https://doi.org/10.1099/ijs.0.048413-0
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