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Abstract

A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterium, designated strain CF5-4, was isolated from iron mining powder. 16S rRNA gene sequence analysis grouped strain CF5-4 in a single cluster with DSM 3133 (97.6 % similarity). The major fatty acids (>5 %) of strain CF5-4 were anteiso-C, anteiso-C A, C, iso-C, iso-C and anteiso-C. The predominant respiratory quinone was MK-10(H) and the genomic DNA G+C content was 74.7 mol%. The major polar lipids were diphosphatidylglycerol and one unidentified phosphoglycolipid. The peptidoglycan type of strain CF5-4 was A4β, containing -Orn–-Ser–-Asp. The cell-wall sugars were rhamnose, fucose, mannose and galactose. The results of DNA–DNA hybridization in combination with the comparison of phenotypic and phylogenetic characteristics among strain CF5-4 and related micro-organisms revealed that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF5-4 ( = KCTC 29134 = CCTCC AB2012198).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31170106)
  • National High Tech Development Program (Award 2012AA101402-3)
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2013-09-01
2024-03-29
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